蜜蜂毒液引起的肌肉、神经肌肉连接处和神经的细胞和亚细胞变化。

A Rodríguez-Acosta, L Peña, H J Finol, M Pulido-Méndez
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引用次数: 0

摘要

从注射蜜蜂粗毒液的动物身上获得的颈-骨肌活检标本准备用于电子显微镜研究。注射蜜蜂毒液6 h后,透射电镜下小鼠肌肉纤维呈不同程度的萎缩,肌纤维间间隙增加。小管和肌浆网分子改变,部分部位仅可见小管碎片和肌纤维间隙增加,纤维不规则性丧失,三联征明显。肌膜下区肌原纤维缺失,线粒体损伤。观察到肌肉节段性坏死和萎缩区。神经肌肉连接改变。突触囊泡数量变化很大,突触间隙不规则。还观察到突触间隙的数量和排列以及自由多聚体的减少,表明再生过程。髓鞘神经分布于轴突或壁空泡区。在我们的实验中,严重肌肉损伤的存在,在突触前区域发现的毒液活动以及在不同时间阶段检测到的神经肌肉连接损伤表明,蜂毒对神经肌肉结构具有高度毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cellular and subcellular changes in muscle, neuromuscular junctions and nerves caused by bee (Apis mellifera) venom.

Biopsy specimens of cervico-scutular muscles obtained from animals injected with bee crude venom were prepared for electron microscopy studies. At 6 h from Apis mellifera venom injection, in mice under transmission electron microscopy, the muscular fibres presented different atrophy levels with increment of the intermyofibrillar spaces. Tubules and sarcoplasmic reticulum elements were altered, in some places only tubular fragments and an increment of the intermyofibrillar spaces were noticed as well as loss of fibre regularity and prominent triads. In subsarcolemma region, areas lacking myofibrils and mitochondria damages were observed. Muscular segmental necrosis and atrophy areas were observed. Neuromuscular junctions were altered. The number of synaptic vesicles was very variable and synaptic clefts showed irregularities. A decrease in the number and arrangement of the synaptic clefts, as well as free polysomes, suggesting regeneration processes, were also observed. The myelinic nerves exhibited in the axon or in the wall vacuolisation areas. The presence of severe muscular lesions, the finding of venom activities in the presynaptic region and the detection of damages in the neuromuscular junctions at different chronological stages of our experiments indicate that the bee venom is highly toxic for neuromuscular structures.

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