Solange Assuncion Villagra Fernandez, Alice Zoghbi Coelho Lobo, Zilda Najjar Prado de Oliveira, Ligia Maria Ichimura Fukumori, Alexandre Marques P rigo, Evandro A Rivitti
{"title":"正常供血者血清中抗核自身抗体的流行。","authors":"Solange Assuncion Villagra Fernandez, Alice Zoghbi Coelho Lobo, Zilda Najjar Prado de Oliveira, Ligia Maria Ichimura Fukumori, Alexandre Marques P rigo, Evandro A Rivitti","doi":"10.1590/s0041-87812003000600005","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To examine the presence of serum antinuclear autoantibodies in a healthy population.</p><p><strong>Methods: </strong>Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion.</p><p><strong>Results: </strong>In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40.</p><p><strong>Conclusion: </strong>The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.</p>","PeriodicalId":76453,"journal":{"name":"Revista do Hospital das Clinicas","volume":"58 6","pages":"315-9"},"PeriodicalIF":0.0000,"publicationDate":"2003-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/s0041-87812003000600005","citationCount":"83","resultStr":"{\"title\":\"Prevalence of antinuclear autoantibodies in the serum of normal blood dornors.\",\"authors\":\"Solange Assuncion Villagra Fernandez, Alice Zoghbi Coelho Lobo, Zilda Najjar Prado de Oliveira, Ligia Maria Ichimura Fukumori, Alexandre Marques P rigo, Evandro A Rivitti\",\"doi\":\"10.1590/s0041-87812003000600005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To examine the presence of serum antinuclear autoantibodies in a healthy population.</p><p><strong>Methods: </strong>Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion.</p><p><strong>Results: </strong>In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40.</p><p><strong>Conclusion: </strong>The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.</p>\",\"PeriodicalId\":76453,\"journal\":{\"name\":\"Revista do Hospital das Clinicas\",\"volume\":\"58 6\",\"pages\":\"315-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1590/s0041-87812003000600005\",\"citationCount\":\"83\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Revista do Hospital das Clinicas\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1590/s0041-87812003000600005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2004/1/28 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista do Hospital das Clinicas","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1590/s0041-87812003000600005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2004/1/28 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
Prevalence of antinuclear autoantibodies in the serum of normal blood dornors.
Objective: To examine the presence of serum antinuclear autoantibodies in a healthy population.
Methods: Serum of 500 normal blood donors between 18 and 60 years of age were tested for the presence of autoantibodies. Antinuclear antibodies were detected by indirect immunofluorescence technique using HEp-2 epithelial cells as the substrate. The presence of dnaN was detected by indirect immunofluorescence technique using Critidia lucillae as the substrate. Anti-SSA (RO), anti-SSB (LA), anti-Sm, and anti-RNP were determined by double radial immunodiffusion.
Results: In the evaluation of the presence of serum antibodies, antinuclear antibodies were detected in 22.6% of the sera. The presence of other antibodies was not significant. The majority of the titers were 1:40.
Conclusion: The presence of autoantibodies is not necessarily pathologic and has to be related to the age group, gender, and clinical condition of the patient.
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