Using the adult zebrafish visual system to study cadherin-2 expression during central nervous system regeneration.

Zebrafish is a good model organism for the study of vertebrate development due to numerous experimental advantages. Here we show that zebrafish are also suitable for the study of cellular and molecular mechanisms underlying tissue regeneration in the vertebrate central nervous system. In this report we describe simple protocols to introduce injuries to the adult zebrafish visual structures and analyze cadherin-2 expression in the regenerating visual system using immunocytochemistry and immunoblotting.