大冢Long-Evans德岛脂肪(OLETF)大鼠- 2型糖尿病模型的血液学改变

Z Kamenov, H Higashino, M Todorova, N Aoki, M Imamura, M Orita, H Yamanishi, A Suzuki, Y Yamanishi, V Christov
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引用次数: 0

摘要

OLETF大鼠的微血管病变并发症与人类糖尿病相似,被认为是2型糖尿病的有用模型。糖尿病患者的红细胞、血小板和白细胞异常被认为在糖尿病微血管病变的发展中起作用。本研究旨在探讨OLETF大鼠是否出现血液学改变以及蔗糖处理对代谢和血液参数的影响。在饮用水中添加30%蔗糖治疗两个月后,测量OLETF大鼠的血液学参数、体重、食物和水摄入量、空腹和非空腹血糖(BG)和糖化血红蛋白(HbA1c)。以未治疗的OLETF大鼠和未患糖尿病的Long-Evans Tokushima Otsuka (LETO)大鼠作为对照。对照组大鼠血小板(Plt)和红细胞(RBC)数量高于对照组,平均细胞体积(MCV)和平均细胞血红蛋白含量(MCH)低于对照组。糖尿病大鼠平均细胞血红蛋白浓度(MCHC)明显升高。蔗糖可以减少食物摄入量和体重,增加空腹血糖和糖化血红蛋白。与对照组相比,红细胞、Hb、Hct、MCV和MCH均下降,而血小板计数明显增加。我们的研究结果表明,与非糖尿病LETO相比,糖尿病OLETF大鼠的红细胞计数、形态学和Plt计数发生了显著变化。蔗糖可加速糖尿病的发展,影响血细胞,诱导红细胞抑制和血小板计数增加,其部分作用在蔗糖停药后仍持续存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hematological alterations in the Otsuka Long-Evans Tokushima fatty (OLETF) rats--a model of type 2 diabetes mellitus.

The OLETF rat develops microangiopathic complications similar to human diabetes and is considered a useful model of Type 2 DM. Erythrocyte, platelet and leucocyte abnormalities described in diabetic patients are thought to play a role in the development of diabetic microangiopathy. This study was designed to investigate whether OLETF rats show hematological alterations and the effect of sucrose treatment on metabolic and blood parameters. Hematological parameters, body weight, food and water intake, fasting and non-fasting blood glucose (BG) and HbA1c were measured in OLETF rats treated for two months with 30% sucrose added to drinking water. Non-treated OLETF rats and non-diabetic Long-Evans Tokushima Otsuka (LETO) rats were used as controls. In the control OLETF rats the number of platelets (Plt) and red blood cells (RBC) was higher, while the mean cell volume (MCV) and the mean cell hemoglobin content (MCH) were lower compared with LETO. Mean cell hemoglobin concentration (MCHC) was significantly higher in the diabetic rats. Sucrose administration decreased food intake and body weight and increased fasting blood glucose and HbA1c. It resulted in a decrease of RBC, Hb, Hct, MCV and MCH compared with control OLETF, while Plt count increased significantly. Our results point to significant alterations in erythrocyte count and morphology and Plt count in diabetic OLETF rats compared with non-diabetic LETO. Sucrose administration accelerated the development of diabetes, affected blood cells inducing the suppression of RBC and an increase in Plt count and some of its effects persisted after sucrose withdrawal.

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