Xiaotong Ma , Haiqiang Jin , Yingying Ren , Zhiyuan Shen , Li Wang , Shitong Zhang , Yuxuan Lu , Shanyue Guan , Shuyun Zhou , Xiaozhong Qu
{"title":"通过姜黄素的三重酶被认为是高效缓解再灌注损伤的酶兴奋剂","authors":"Xiaotong Ma , Haiqiang Jin , Yingying Ren , Zhiyuan Shen , Li Wang , Shitong Zhang , Yuxuan Lu , Shanyue Guan , Shuyun Zhou , Xiaozhong Qu","doi":"10.1016/j.cej.2022.136029","DOIUrl":null,"url":null,"abstract":"<div><p>For alleviating ischemic reperfusion injury, reasonable design mimic multi-functional nanozyme can simultaneously capture ROS and inhibit inflammatory factors, which plays an important role. However, the low efficiency of natural enzyme and their ability to capture only a single species limit their effectiveness. Therefore, additional materials to capture other ROS are highly needed. These motivates us to construct a nanozyme <em>via</em> hybridize/coupled curcumin (CUR) with ZnCe Layered Rare-earth Hydroxide (ZnCe-LRH) to achieve highly efficient ROS scavenging efficiency and the multiple ROS capture (denoted as CUR/ZnCe-LRH). Ce element was highly dispersed on the LRH layer, and CUR further coordinated with Zn on the layer to form a stable nanozyme structure. This mimic tripartite nanozyme can not only simulate superoxide dismutase (SOD) and catalase (CAT) activity to capture •O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>, but also arrest •OH within a short period of time, which can be regarded as the tripartite nanozyme. Density functional theory (DFT) calculations can further confirm the high efficient ROS scavenging mechanism by the CUR/ZnCe-LRH systems. <em>In vivo</em> results can further probe that this CUR/ZnCe-LRH can also suppress inflammation- and immune response–induced injury, thus achieving good prevention and treatment in neuroprotective therapy. The infarct area can reduce by 78% after the reperfusion therapy and the neurological deficit score can reduce from 3.50 to 0.67. This strategy provided a novel multifunctional enzyme mimetic for ischemia–reperfusion therapy and clarifies the application mechanism of neuroprotection against ischemia–reperfusion injury.</p></div>","PeriodicalId":270,"journal":{"name":"Chemical Engineering Journal","volume":null,"pages":null},"PeriodicalIF":13.3000,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":"{\"title\":\"A tripartite-enzyme via curcumin regarded as zymoexciter towards highly efficient relieving reperfusion injury\",\"authors\":\"Xiaotong Ma , Haiqiang Jin , Yingying Ren , Zhiyuan Shen , Li Wang , Shitong Zhang , Yuxuan Lu , Shanyue Guan , Shuyun Zhou , Xiaozhong Qu\",\"doi\":\"10.1016/j.cej.2022.136029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>For alleviating ischemic reperfusion injury, reasonable design mimic multi-functional nanozyme can simultaneously capture ROS and inhibit inflammatory factors, which plays an important role. However, the low efficiency of natural enzyme and their ability to capture only a single species limit their effectiveness. Therefore, additional materials to capture other ROS are highly needed. These motivates us to construct a nanozyme <em>via</em> hybridize/coupled curcumin (CUR) with ZnCe Layered Rare-earth Hydroxide (ZnCe-LRH) to achieve highly efficient ROS scavenging efficiency and the multiple ROS capture (denoted as CUR/ZnCe-LRH). Ce element was highly dispersed on the LRH layer, and CUR further coordinated with Zn on the layer to form a stable nanozyme structure. This mimic tripartite nanozyme can not only simulate superoxide dismutase (SOD) and catalase (CAT) activity to capture •O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>, but also arrest •OH within a short period of time, which can be regarded as the tripartite nanozyme. Density functional theory (DFT) calculations can further confirm the high efficient ROS scavenging mechanism by the CUR/ZnCe-LRH systems. <em>In vivo</em> results can further probe that this CUR/ZnCe-LRH can also suppress inflammation- and immune response–induced injury, thus achieving good prevention and treatment in neuroprotective therapy. The infarct area can reduce by 78% after the reperfusion therapy and the neurological deficit score can reduce from 3.50 to 0.67. This strategy provided a novel multifunctional enzyme mimetic for ischemia–reperfusion therapy and clarifies the application mechanism of neuroprotection against ischemia–reperfusion injury.</p></div>\",\"PeriodicalId\":270,\"journal\":{\"name\":\"Chemical Engineering Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":13.3000,\"publicationDate\":\"2022-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemical Engineering Journal\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1385894722015273\",\"RegionNum\":1,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, CHEMICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1385894722015273","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
A tripartite-enzyme via curcumin regarded as zymoexciter towards highly efficient relieving reperfusion injury
For alleviating ischemic reperfusion injury, reasonable design mimic multi-functional nanozyme can simultaneously capture ROS and inhibit inflammatory factors, which plays an important role. However, the low efficiency of natural enzyme and their ability to capture only a single species limit their effectiveness. Therefore, additional materials to capture other ROS are highly needed. These motivates us to construct a nanozyme via hybridize/coupled curcumin (CUR) with ZnCe Layered Rare-earth Hydroxide (ZnCe-LRH) to achieve highly efficient ROS scavenging efficiency and the multiple ROS capture (denoted as CUR/ZnCe-LRH). Ce element was highly dispersed on the LRH layer, and CUR further coordinated with Zn on the layer to form a stable nanozyme structure. This mimic tripartite nanozyme can not only simulate superoxide dismutase (SOD) and catalase (CAT) activity to capture •O2− and H2O2, but also arrest •OH within a short period of time, which can be regarded as the tripartite nanozyme. Density functional theory (DFT) calculations can further confirm the high efficient ROS scavenging mechanism by the CUR/ZnCe-LRH systems. In vivo results can further probe that this CUR/ZnCe-LRH can also suppress inflammation- and immune response–induced injury, thus achieving good prevention and treatment in neuroprotective therapy. The infarct area can reduce by 78% after the reperfusion therapy and the neurological deficit score can reduce from 3.50 to 0.67. This strategy provided a novel multifunctional enzyme mimetic for ischemia–reperfusion therapy and clarifies the application mechanism of neuroprotection against ischemia–reperfusion injury.
期刊介绍:
The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.