腹腔注射[14C]-α-生育酚大鼠肝脏代谢产物的研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects Pub Date : 1964-12-02 DOI:10.1016/0926-6542(64)90031-9

P.A. Plack , J.G. Bieri

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引用次数: 20

摘要

喂养1 ~ 5个月缺乏维生素e的大鼠，腹腔注射1 mg [14C]-α-生育酚。48 h后处死，取其生命，用薄层色谱法和柱层析法分析α-生育酚及其代谢产物。在回收的放射性物质中，约80%以未改变的α-生育酚的形式存在，11%以极性更强的化合物存在，可能是α-生育酚醌。没有获得二聚体存在的明确证据。对照实验表明，用乙腈水溶液在薄层板上色谱α-生育酚时，会产生相当数量的极性物质。

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Metabolic products of α-tocopherol in the livers of rats given intraperitoneal injections of [14C]-α-tocopherol

Rats fed a vitamin E-deficient diet for 1–5 months were given intraperitoneal injections of 1 mg [¹⁴C]-α-tocopherol. After 48 h thye were killed and their lives removed and analyzed by thin-layer and column chromatography for α-tocopherol and metabolic products. Of the recovered radioactivity, about 80% was in the form of uncahnged α-tocopherol and 11% was present as a more polar compound, probably α-tocopherylquinone. No definitive evidence was obtained for the presence of a dimer. Control experiments showed that chromatography of α-tocopherol on thin-layer plates, with aqueous acetonitrile, gave rise to considerable amounts of polar material.

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects

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