{"title":"嘌呤霉素对培养脂肪组织的影响及其对地塞米松、胰岛素和肾上腺素的反应","authors":"John N. Fain","doi":"10.1016/0926-6542(64)90023-X","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Puromycin (10<sup>−4</sup> M), puromycin aminonucleoside (10<sup>−4</sup> M) and dexamethasone (4·10<sup>−8</sup> M) decreased the uptake and conversion of glucose to CO<sub>2</sub>, glyceride-glycerol and fatty acid by incubated rat-adipose tissue. These substances had little effect on the metabolism of fructose or pyruvate by adipose tissue. Puromycin blocked or decreased the incorporation of glucose, fructose, and pyruvate carbon into protein; aminonucleoside and dexamethasone had little effect.</p></span></li><li><span>2.</span><span><p>2. Puromycin blocked the stimulation of protein synthesis by insulin but did not alter the other actions of insulin or the effects of dexamethasone and epinephrine on adipose tissue.</p></span></li><li><span>3.</span><span><p>3. Insulin decreased the conversion of pyruvate to lactate and increased its conversion to fatty acid in the presence and the absence of puromycin.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1964-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90023-X","citationCount":"27","resultStr":"{\"title\":\"Effect of puromycin on incubated adipose tissue and its response to dexamethasone, insulin, and epinephrine\",\"authors\":\"John N. Fain\",\"doi\":\"10.1016/0926-6542(64)90023-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Puromycin (10<sup>−4</sup> M), puromycin aminonucleoside (10<sup>−4</sup> M) and dexamethasone (4·10<sup>−8</sup> M) decreased the uptake and conversion of glucose to CO<sub>2</sub>, glyceride-glycerol and fatty acid by incubated rat-adipose tissue. These substances had little effect on the metabolism of fructose or pyruvate by adipose tissue. Puromycin blocked or decreased the incorporation of glucose, fructose, and pyruvate carbon into protein; aminonucleoside and dexamethasone had little effect.</p></span></li><li><span>2.</span><span><p>2. Puromycin blocked the stimulation of protein synthesis by insulin but did not alter the other actions of insulin or the effects of dexamethasone and epinephrine on adipose tissue.</p></span></li><li><span>3.</span><span><p>3. Insulin decreased the conversion of pyruvate to lactate and increased its conversion to fatty acid in the presence and the absence of puromycin.</p></span></li></ul></div>\",\"PeriodicalId\":100171,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-12-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6542(64)90023-X\",\"citationCount\":\"27\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/092665426490023X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092665426490023X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of puromycin on incubated adipose tissue and its response to dexamethasone, insulin, and epinephrine
1.
1. Puromycin (10−4 M), puromycin aminonucleoside (10−4 M) and dexamethasone (4·10−8 M) decreased the uptake and conversion of glucose to CO2, glyceride-glycerol and fatty acid by incubated rat-adipose tissue. These substances had little effect on the metabolism of fructose or pyruvate by adipose tissue. Puromycin blocked or decreased the incorporation of glucose, fructose, and pyruvate carbon into protein; aminonucleoside and dexamethasone had little effect.
2.
2. Puromycin blocked the stimulation of protein synthesis by insulin but did not alter the other actions of insulin or the effects of dexamethasone and epinephrine on adipose tissue.
3.
3. Insulin decreased the conversion of pyruvate to lactate and increased its conversion to fatty acid in the presence and the absence of puromycin.
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