{"title":"用于质膜外部成分的荧光标记","authors":"A.H. Maddy","doi":"10.1016/0926-6577(64)90194-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The synthesis of a new fluorescent reagent (4-acetamido,4′-isothiocyanostilbene-2,2′-disulphonic acid) for the specific labelling of the outer components of the plasma membrane is described.</p></span></li><li><span>2.</span><span><p>2. The reaction of the reagent with ox erythrocytes is examined. It is concluded that the reagent reacts with a fixed number of sites on the surface of the cell.</p></span></li><li><span>3.</span><span><p>3. The problems of designing reagents of this type, and the nature of the reaction between the stilbene isothiocyanate and erythrocytes are discussed.</p></span></li></ul></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":"88 2","pages":"Pages 390-399"},"PeriodicalIF":0.0000,"publicationDate":"1964-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90194-9","citationCount":"203","resultStr":"{\"title\":\"A fluorescent label for the outer components of the plasma membrane\",\"authors\":\"A.H. Maddy\",\"doi\":\"10.1016/0926-6577(64)90194-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. The synthesis of a new fluorescent reagent (4-acetamido,4′-isothiocyanostilbene-2,2′-disulphonic acid) for the specific labelling of the outer components of the plasma membrane is described.</p></span></li><li><span>2.</span><span><p>2. The reaction of the reagent with ox erythrocytes is examined. It is concluded that the reagent reacts with a fixed number of sites on the surface of the cell.</p></span></li><li><span>3.</span><span><p>3. The problems of designing reagents of this type, and the nature of the reaction between the stilbene isothiocyanate and erythrocytes are discussed.</p></span></li></ul></div>\",\"PeriodicalId\":100169,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"volume\":\"88 2\",\"pages\":\"Pages 390-399\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-09-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6577(64)90194-9\",\"citationCount\":\"203\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926657764901949\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926657764901949","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A fluorescent label for the outer components of the plasma membrane
1.
1. The synthesis of a new fluorescent reagent (4-acetamido,4′-isothiocyanostilbene-2,2′-disulphonic acid) for the specific labelling of the outer components of the plasma membrane is described.
2.
2. The reaction of the reagent with ox erythrocytes is examined. It is concluded that the reagent reacts with a fixed number of sites on the surface of the cell.
3.
3. The problems of designing reagents of this type, and the nature of the reaction between the stilbene isothiocyanate and erythrocytes are discussed.
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