铜绿假单胞菌肽肽水解酶IV.旋光色散和氨基酸组成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects Pub Date : 1964-11-22 DOI:10.1016/0926-6569(64)90194-4

Kazuyuki Morihara, Nobuo Yoshida, Kaoru Kuriyama

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引用次数: 11

摘要

研究了铜绿假单胞菌IFO 3080结晶蛋白酶(肽肽水解酶)的一些理化性质。[α]D为−30°。由旋光色散得出的λc为227.6 μ， b0为−30。螺旋含量由b0计算为5.2%。该蛋白酶的氨基酸组成为:天冬氨酸66、苏氨酸24、丝氨酸42、谷氨酸35、脯氨酸11、甘氨酸65、丙氨酸58、缬氨酸25、异亮氨酸17、亮氨酸37、酪氨酸21、苯丙氨酸20、赖氨酸16、组氨酸6、精氨酸7、色氨酸6。含硫氨基酸如胱氨酸、半胱氨酸和蛋氨酸未检出。

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Pseudomonas aeruginosa peptide peptidohydrolase IV. Optical rotatory dispersion and amino acid composition

Some physico-chemical properties of the crystalline proteinase (peptide peptidohydrolase) from Pseudomonas aeruginosa IFO 3080 were studied. The [α]_D was −30°. The λ_c was 227.6 mμ and b₀ was −30, derived from the optical rotatory dispersion. The helical content was calculated as 5.2% from the value of b₀. The amino acid composition of the proteinase was as follows: aspartic acid 66, threonine 24, serine 42, glutamic acid 35, proline 11, glycine 65, alanine 58, valine 25, isoleucine 17, leucine 37, tyrosine 21, phenylalanine 20, lysine 16, histidine 6, arginine 7, tryptophan 6. Amino acids containing sulfur, such as cystine, cysteine and methionine, were not detected.

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects

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