{"title":"来自三磷酸的32P在红细胞膜内与多磷酸肌醇[γ-32P]腺苷和磷脂酸结合","authors":"Lowell E. Hokin , Mabel R. Hokin","doi":"10.1016/0926-6542(64)90126-X","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. Erythrocyte ghosts incubated with [γ-<sup>32</sup>P]ATP incorporate radioactivity into diphosphoinositide, triphosphoinositide, phosphatidic acid, and into at least one unidentified lipid.</p></span></li><li><span>2.</span><span><p>2. Over 90% of the radioactivity incorporated into the polyphosphoinositides is in the monoesterified phosphates.</p></span></li><li><span>3.</span><span><p>3. Exogenous phosphatidyl inositol increases the labeling in diphosphoinositide by about 30% and in the unidentified compound by about 200%. The labeling in triphosphoinositide is inhibited.</p></span></li><li><span>4.</span><span><p>4. Kinetic studies of the labeling of the polyphosphoinsitides fail to provide evidence that the exchange of phosphate in the monoesterified positions is an intermediate reaction in the magnesium-dependent, sodium and potassium-activated ATPase.</p></span></li></ul></div>","PeriodicalId":100171,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","volume":"84 5","pages":"Pages 563-575"},"PeriodicalIF":0.0000,"publicationDate":"1964-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6542(64)90126-X","citationCount":"77","resultStr":"{\"title\":\"The incorporation of 32P from triphosphate into polyphosphoinositides [γ-32P]adenosine and phosphatidic acid in erythrocyte membranes\",\"authors\":\"Lowell E. Hokin , Mabel R. Hokin\",\"doi\":\"10.1016/0926-6542(64)90126-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. Erythrocyte ghosts incubated with [γ-<sup>32</sup>P]ATP incorporate radioactivity into diphosphoinositide, triphosphoinositide, phosphatidic acid, and into at least one unidentified lipid.</p></span></li><li><span>2.</span><span><p>2. Over 90% of the radioactivity incorporated into the polyphosphoinositides is in the monoesterified phosphates.</p></span></li><li><span>3.</span><span><p>3. Exogenous phosphatidyl inositol increases the labeling in diphosphoinositide by about 30% and in the unidentified compound by about 200%. The labeling in triphosphoinositide is inhibited.</p></span></li><li><span>4.</span><span><p>4. Kinetic studies of the labeling of the polyphosphoinsitides fail to provide evidence that the exchange of phosphate in the monoesterified positions is an intermediate reaction in the magnesium-dependent, sodium and potassium-activated ATPase.</p></span></li></ul></div>\",\"PeriodicalId\":100171,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"volume\":\"84 5\",\"pages\":\"Pages 563-575\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6542(64)90126-X\",\"citationCount\":\"77\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/092665426490126X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092665426490126X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The incorporation of 32P from triphosphate into polyphosphoinositides [γ-32P]adenosine and phosphatidic acid in erythrocyte membranes
1.
1. Erythrocyte ghosts incubated with [γ-32P]ATP incorporate radioactivity into diphosphoinositide, triphosphoinositide, phosphatidic acid, and into at least one unidentified lipid.
2.
2. Over 90% of the radioactivity incorporated into the polyphosphoinositides is in the monoesterified phosphates.
3.
3. Exogenous phosphatidyl inositol increases the labeling in diphosphoinositide by about 30% and in the unidentified compound by about 200%. The labeling in triphosphoinositide is inhibited.
4.
4. Kinetic studies of the labeling of the polyphosphoinsitides fail to provide evidence that the exchange of phosphate in the monoesterified positions is an intermediate reaction in the magnesium-dependent, sodium and potassium-activated ATPase.
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