关于来自不同生物体的氨基酸核糖核酸合成酶缺乏统一的特异性

K. Bruce Jacobson, S. Nishimura , W.Edgar Barnett, Rusty J. Mans , P. Cammarano , G. David Novelli
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引用次数: 21

摘要

利用同源的(大肠杆菌)氨基酰基RNA合成酶(氨基酸-RNA连接酶)制备了11个大肠杆菌氨基酰基RNA。并与三种异种合成酶(小鼠肝、玉米、粗神经孢子虫)合成的氨基酸基RNA进行了色谱行为比较。在某些情况下,含有单一氨基酸和单一s-RNA混合物的两种酶制剂的产物在色谱上是可分离的,这表明酶从不同来源选择的s-RNA分子在化学上存在差异。结果表明,每种生物同源氨基酰化的11种氨基酰RNA的色谱迁移率的相对顺序各不相同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
On the lack of a uniform specificity of aminoacyl ribonucleic acid synthetases from different organisms

11 Aminoacyl RNA's of Escherichia coli were prepared using the homologous (E. coli) aminoacyl RNA synthetases (amino acid-RNA ligases). Their chromatographic behavior was compared to those aminoacyl RNA's prepared using three heterologous (mouse liver, maize, Neurospora crassa) synthetases. In some cases the products of two enzyme preparations with a single amino acid and a single s-RNA mixture are chromatographically separable, indicating a chemical difference in the s-RNA molecules selected by the enzymes from different sources.

The relative order of chromatographic mobilities of the 11 aminoacyl RNA's from each organism, homologously aminoacylated, were shown to vary.

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