电镜和细胞化学染色用福尔马林固定物的研究。

S J HOLT, R M HICKS
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引用次数: 230

摘要

研究了固定在含4%甲醛溶液中的大鼠肝脏的精细结构、磷脂以及酸性磷酸酶和酯酶的活性。对甲基丙烯酸酯包埋制剂的检查表明,含钙固定剂导致精细结构保存不良,而如果通过添加蔗糖适当调整溶液的滋补性,则经维罗那纳处理或磷酸盐缓冲的福尔马林可以获得极好的结果。添加了茴香的甲醛-磷酸盐会导致细胞形态的恶化。磷脂几乎被定量地保留在固定在福尔马林中的组织中,以及在磷酸盐、碰撞碱或三乙醇胺缓冲的福尔马林中。大约50%的酸性磷酸酶和酯酶的活性在0-2摄氏度的固定液中暴露24小时后得以保存,根据细胞化学染色结果判断,酶和磷脂的分布没有被任何这些福尔马林溶液改变。考虑到固定组织的形态和生化完整性,4%的甲醛,在pH值为7.2时用0.067 M的磷酸盐缓冲,含有7.5%的蔗糖,是最适合细胞化学染色和电子显微镜研究的固定剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies on formalin fixation for electron microscopy and cytochemical staining purposes.

A study has been made of the preservation of fine structure, phospholipids, and the activity of acid phosphatase and esterase in rat liver fixed in various solutions containing 4 per cent formaldehyde. Examination of methacrylate-embedded preparations shows that calcium-containing fixatives result in poor preservation of fine structure, whereas veronal-treated or phosphate-buffered formalin gives excellent results if the tonicity of the solutions is suitably adjusted by addition of sucrose. Formol-phosphate, to which Versene has been added, causes deterioration of cellular morphology. Phospholipids are retained almost quantitatively in tissue fixed in formol-calcium, and in phosphate-, collidine-, or triethanolamine-buffered formalin. About 50 per cent of the activity of acid phosphatase and esterase are preserved after 24 hours exposure to these fixatives at 0-2 degrees C, and the distributions of the enzymes and of phospholipids, as judged by cytochemical staining results, are not altered by any of these formalin solutions. Consideration of the morphological and biochemical integrity of the fixed tissue suggests that 4 per cent formaldehyde, buffered at pH 7.2 with 0.067 M phosphate, and containing 7.5 per cent sucrose, is the most suitable of the fixatives for combined cytochemical staining and electron microscopical studies.

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