DNA链烷的制备及原子力显微镜对其拓扑结构的观察。

Nucleic acids symposium series Pub Date : 2000-01-01 DOI:10.1093/nass/44.1.229

H Yamaguchi, K Kubota, A Harada

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引用次数: 9

摘要

在有缺口DNA存在的情况下，用T4 DNA连接酶与线性DNA反应制备了DNA链链烷。在室温和环境气氛下，利用原子力显微镜可以清晰地观察到DNA链链烷和大型环状DNA的单分子图像。

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Preparation of DNA catenanes and observation of their topological structures by atomic force microscopy.

DNA catenanes have been prepared by the reaction of T4 DNA ligase with linear DNA in the presence of nicked DNA. Single molecular images of DNA catenanes and large circular DNAs have been clearly observed by AFM using a tapping mode at room temperature and in an ambient atmosphere.

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Nucleic acids symposium series

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