DNA结合肽的结构和亲和力。

T Kubo, K Yokoyama, R Ueki, S Abe, K Goto, T Niidome, H Aoyagi, K Iwakuma, S Ando, S Ono, M Fujii
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引用次数: 7

摘要

合成了天然DNA/RNA结合蛋白中最常见的-螺旋、-转、反平行- -片和- -发夹结构的人工肽,并通过紫外熔化实验、CD光谱和SPR测量在双链或三链DNA存在或不存在的情况下检测了它们的特征特征。结果表明,由于多肽的特殊二级结构和疏水面的正电荷所产生的两亲性在与DNA的相互作用中起着重要的作用,并且阳离子两亲性多肽对杂化双链和三链具有很强的稳定性。还发现这些肽可以保护dsDNA免受DNase 1的消化。这些结果表明,本研究合成的结构设计的两亲肽可以成为反义和抗原策略的有力工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Structure and affinity of DNA binding peptides.

Artificial peptides designed to form alpha-helical, beta-turn, antiparallel beta-sheet and beta-hairpin structures which are among the motifs most frequently found in natural DNA/RNA binding proteins were synthesized and their characteristic features were examined in the presence or absence of double or triple stranded DNA by means of UV melting experiments, CD spectra, SPR measurements. It was revealed that amphiphilic character arising from the specific secondary structures and positive charge in the hydrophobic face of peptides played an important role in the interaction with DNA, and that hybrid duplex and triplex were intensively stabilized by the cationic amphiphilic peptides. It was also found that these peptides could protect dsDNA against DNase 1 digestion. These results indicate that structurally designed amphiphilic peptides synthesized in the present study can be powerful tools for antisense and antigene strategies.

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