脊椎动物mrna翻译起始信号的定量分析:+4(-)+6位点核苷酸对翻译起始效率的影响。

Y Iida, D Kanagu
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引用次数: 2

摘要

关于脊椎动物mrna的翻译起始信号,已经提出了一个共识序列(GCC)GCC(a or G) CCATGG,但实际的起始序列或多或少存在差异。Kozak监测核糖体对第一和第二ATG密码子的选择,作为在第一个ATG密码子+4和+6位置引入突变的函数。具有G at +4的密码子强烈增强了第一个ATG密码子的选择。然而,ATG密码子识别不受大多数位置和+6突变的影响。我们的量化分析很好地理解了这些数据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification analysis of translation initiation signal in vertebrate mRNAs: effect of nucleotides at positions +4(-)+6 upon efficiency of translation initiation.
Concerning the translation initiation signal in vertebrate mRNAs, a consensus, sequence, (GCC)GCC(A or G) CCATGG, has been proposed, but actual initiation sequences differ from it in a greater or lesser degree. Kozak monitored selection by ribosomes of the first versus second ATG codons as a function of mutations introduced at positions +4, and +6 of the first ATG codon. Codons possessing G at +4 strongly enhanced selection of the first ATG codon. However, ATG codon recognition was unaffected by most mutations in positions and +6. These data were well understood by our quantification analysis.
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