从纺锤树(Euonymus europaeus L.)树皮中提取的一种hevein型抗菌肽Ee-CBP。

Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen) Pub Date : 2002-01-01

K P B Van den Bergh, E J M Van Damme, W J Peumans, J Coosemans

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摘要

从纺锤树(Euonymus europaeus L.)的树皮中分离得到一种hevein型抗菌肽Ee-CBP。4992.5 Da蛋白对五种不同的真菌病原真菌具有很强的抗真菌活性。琼脂扩散法和微滴板法抑制灰葡萄孢生长所需的浓度分别为5微克/毫升和1微克/毫升。对比实验进一步表明，Ee-CBP对同一种真菌的抑菌活性高于苋属种子中Ac-AMP2的抑菌活性。

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Ee-CBP, a hevein-type antimicrobial peptide from bark of the spindle tree (Euonymus europaeus L.).

Ee-CBP, a hevein-type antimicrobial peptide was isolated from the bark of the spindle tree (Euonymus europaeus L.). This 4992.5 Da protein exhibited a very strong antifungal activity against five different fytopathogenic fungi that were tested. Concentrations required to inhibit the growth of Botrytis cinerea in agar diffusion assays and microtiterplate assays were 5 micrograms/ml and 1 microgram/ml, respectively. Comparative tests further indicated that Ee-CBP is a more potent antifungal protein than Ac-AMP2, an antimicrobial peptide from seeds of Amaranthus caudatus L. when tested with the same fungus.

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Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)

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