取样技术对腹膜纤溶活性测定的影响。

J Neudecker, T Junghans, S Ziemer, W Raue, W Schwenk
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引用次数: 4

摘要

目的:探讨腹膜取样技术对测定纤溶能力的影响。设计:临床研究。地点:德国大学医院。研究对象:从10例择期结肠切除术患者中取出40例腹膜活检标本。干预措施:采用活检打孔机(n = 20)或手工用镊子和剪刀(n = 20)采集腹膜活检标本。主要结果测量:活检打孔法和手工法取样的纤维蛋白溶解活性的一致程度。主要终点:腹膜组织纤溶酶原激活物(t-PA)活性。次要终点-腹膜组织纤溶酶原激活剂浓度,以及1型纤溶酶原激活剂抑制(PAT-1)的浓度和活性。结果:采用Bland和Altman法评价组内一致性和组间一致性程度。使用同一ELISA试剂盒对同一样品重复测定t-PA和PAI-1浓度与活性的相关性高(r = 0.93-0.99, p < 0.01)。t-PA活性和浓度组间相关性较差(r= 0.60和0.66,p < 0.01), PAI-1浓度和活性组间无相关性(r= 0.6和0.1,p = 0.2和0.9)。两组之间的平均差异在-27%到-4.8%之间。结论:取样技术对腹膜纤溶活性测定有显著影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of the sampling technique on the measurement of peritoneal fibrinolytic activity.

Objective: To establish the influence of the peritoneal sampling technique on the measurement of fibrinolytic capacity.

Design: Clinical study.

Setting: University hospital, Germany.

Subjects: 40 peritoneal biopsy specimens were taken from 10 patients who were having elective colorectal resections.

Interventions: Peritoneal biopsy specimens were taken either with a biopsy punch (n = 20) or manually with forceps and scissors (n = 20).

Main outcome measures: Extent of agreement in fibrinolytic activities between specimens taken with biopsy punch and manually. Major endpoint-peritoneal tissue plasminogen activator (t-PA) activity. Minor endpoints-peritoneal tissue plasminogen activator concentration, and concentration and activity of plasminogen activator inhibitior type 1 (PAT-1).

Results: Intra-assay agreement and the extent of agreement between the groups were evaluated by the method of Bland and Altman. Correlation of repeated measurements of t-PA and PAI-1 concentrations and activities from the same sample using the same ELISA kit was high (r = 0.93-0.99, p < 0.01). t-PA activities and concentrations between the groups correlated poorly (r= 0.60 and 0.66, p < 0.01) while no correlation at all was seen for PAI-1 concentration and activity between the groups (r = 0.6 and 0.1, p = 0.2 and 0.9). The mean differences between the groups ranged from -27% to -4.8%.

Conclusion: The sampling technique considerably affects the measurement of peritoneal fibrinolytic activity.

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