J Neudecker, T Junghans, S Ziemer, W Raue, W Schwenk
{"title":"取样技术对腹膜纤溶活性测定的影响。","authors":"J Neudecker, T Junghans, S Ziemer, W Raue, W Schwenk","doi":"10.1080/11024150201680012","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To establish the influence of the peritoneal sampling technique on the measurement of fibrinolytic capacity.</p><p><strong>Design: </strong>Clinical study.</p><p><strong>Setting: </strong>University hospital, Germany.</p><p><strong>Subjects: </strong>40 peritoneal biopsy specimens were taken from 10 patients who were having elective colorectal resections.</p><p><strong>Interventions: </strong>Peritoneal biopsy specimens were taken either with a biopsy punch (n = 20) or manually with forceps and scissors (n = 20).</p><p><strong>Main outcome measures: </strong>Extent of agreement in fibrinolytic activities between specimens taken with biopsy punch and manually. Major endpoint-peritoneal tissue plasminogen activator (t-PA) activity. Minor endpoints-peritoneal tissue plasminogen activator concentration, and concentration and activity of plasminogen activator inhibitior type 1 (PAT-1).</p><p><strong>Results: </strong>Intra-assay agreement and the extent of agreement between the groups were evaluated by the method of Bland and Altman. Correlation of repeated measurements of t-PA and PAI-1 concentrations and activities from the same sample using the same ELISA kit was high (r = 0.93-0.99, p < 0.01). t-PA activities and concentrations between the groups correlated poorly (r= 0.60 and 0.66, p < 0.01) while no correlation at all was seen for PAI-1 concentration and activity between the groups (r = 0.6 and 0.1, p = 0.2 and 0.9). The mean differences between the groups ranged from -27% to -4.8%.</p><p><strong>Conclusion: </strong>The sampling technique considerably affects the measurement of peritoneal fibrinolytic activity.</p>","PeriodicalId":22411,"journal":{"name":"The European journal of surgery = Acta chirurgica","volume":"168 11","pages":"635-40"},"PeriodicalIF":0.0000,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Influence of the sampling technique on the measurement of peritoneal fibrinolytic activity.\",\"authors\":\"J Neudecker, T Junghans, S Ziemer, W Raue, W Schwenk\",\"doi\":\"10.1080/11024150201680012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To establish the influence of the peritoneal sampling technique on the measurement of fibrinolytic capacity.</p><p><strong>Design: </strong>Clinical study.</p><p><strong>Setting: </strong>University hospital, Germany.</p><p><strong>Subjects: </strong>40 peritoneal biopsy specimens were taken from 10 patients who were having elective colorectal resections.</p><p><strong>Interventions: </strong>Peritoneal biopsy specimens were taken either with a biopsy punch (n = 20) or manually with forceps and scissors (n = 20).</p><p><strong>Main outcome measures: </strong>Extent of agreement in fibrinolytic activities between specimens taken with biopsy punch and manually. Major endpoint-peritoneal tissue plasminogen activator (t-PA) activity. Minor endpoints-peritoneal tissue plasminogen activator concentration, and concentration and activity of plasminogen activator inhibitior type 1 (PAT-1).</p><p><strong>Results: </strong>Intra-assay agreement and the extent of agreement between the groups were evaluated by the method of Bland and Altman. Correlation of repeated measurements of t-PA and PAI-1 concentrations and activities from the same sample using the same ELISA kit was high (r = 0.93-0.99, p < 0.01). t-PA activities and concentrations between the groups correlated poorly (r= 0.60 and 0.66, p < 0.01) while no correlation at all was seen for PAI-1 concentration and activity between the groups (r = 0.6 and 0.1, p = 0.2 and 0.9). The mean differences between the groups ranged from -27% to -4.8%.</p><p><strong>Conclusion: </strong>The sampling technique considerably affects the measurement of peritoneal fibrinolytic activity.</p>\",\"PeriodicalId\":22411,\"journal\":{\"name\":\"The European journal of surgery = Acta chirurgica\",\"volume\":\"168 11\",\"pages\":\"635-40\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The European journal of surgery = Acta chirurgica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/11024150201680012\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The European journal of surgery = Acta chirurgica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/11024150201680012","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Influence of the sampling technique on the measurement of peritoneal fibrinolytic activity.
Objective: To establish the influence of the peritoneal sampling technique on the measurement of fibrinolytic capacity.
Design: Clinical study.
Setting: University hospital, Germany.
Subjects: 40 peritoneal biopsy specimens were taken from 10 patients who were having elective colorectal resections.
Interventions: Peritoneal biopsy specimens were taken either with a biopsy punch (n = 20) or manually with forceps and scissors (n = 20).
Main outcome measures: Extent of agreement in fibrinolytic activities between specimens taken with biopsy punch and manually. Major endpoint-peritoneal tissue plasminogen activator (t-PA) activity. Minor endpoints-peritoneal tissue plasminogen activator concentration, and concentration and activity of plasminogen activator inhibitior type 1 (PAT-1).
Results: Intra-assay agreement and the extent of agreement between the groups were evaluated by the method of Bland and Altman. Correlation of repeated measurements of t-PA and PAI-1 concentrations and activities from the same sample using the same ELISA kit was high (r = 0.93-0.99, p < 0.01). t-PA activities and concentrations between the groups correlated poorly (r= 0.60 and 0.66, p < 0.01) while no correlation at all was seen for PAI-1 concentration and activity between the groups (r = 0.6 and 0.1, p = 0.2 and 0.9). The mean differences between the groups ranged from -27% to -4.8%.
Conclusion: The sampling technique considerably affects the measurement of peritoneal fibrinolytic activity.