{"title":"羟基磷灰石批洗脱法快速分离核蛋白及其复合物的方法。","authors":"A Zagariya, S Khrapunov, W Zacharias","doi":"10.1016/0021-9673(93)83311-f","DOIUrl":null,"url":null,"abstract":"<p><p>A new procedure for the separation and purification of nuclear proteins and their complexes by batch elution from hydroxyapatite is presented. This method allows to isolate such proteins with different basic character faster and more efficiently than procedures using column chromatography, while showing high selectivity, sensitivity, simplicity, mild conditions of purification, reproducibility and protein stability.</p>","PeriodicalId":15508,"journal":{"name":"Journal of chromatography","volume":"648 1","pages":"275-8"},"PeriodicalIF":0.0000,"publicationDate":"1993-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0021-9673(93)83311-f","citationCount":"4","resultStr":"{\"title\":\"Rapid method for the fractionation of nuclear proteins and their complexes by batch elution from hydroxyapatite.\",\"authors\":\"A Zagariya, S Khrapunov, W Zacharias\",\"doi\":\"10.1016/0021-9673(93)83311-f\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A new procedure for the separation and purification of nuclear proteins and their complexes by batch elution from hydroxyapatite is presented. This method allows to isolate such proteins with different basic character faster and more efficiently than procedures using column chromatography, while showing high selectivity, sensitivity, simplicity, mild conditions of purification, reproducibility and protein stability.</p>\",\"PeriodicalId\":15508,\"journal\":{\"name\":\"Journal of chromatography\",\"volume\":\"648 1\",\"pages\":\"275-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1993-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0021-9673(93)83311-f\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of chromatography\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/0021-9673(93)83311-f\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of chromatography","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/0021-9673(93)83311-f","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Rapid method for the fractionation of nuclear proteins and their complexes by batch elution from hydroxyapatite.
A new procedure for the separation and purification of nuclear proteins and their complexes by batch elution from hydroxyapatite is presented. This method allows to isolate such proteins with different basic character faster and more efficiently than procedures using column chromatography, while showing high selectivity, sensitivity, simplicity, mild conditions of purification, reproducibility and protein stability.
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