超临界流体分离生物合成多不饱和脂肪酸(PUFA)。

Li Wang, Sivakumar Muttucumaru
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引用次数: 5

摘要

采用超临界流体分离生物合成多不饱和脂肪酸。该多不饱和脂肪酸是由一株分离自海鱼的海洋细菌WL-1021合成的。WL-1021具有在人工培养基中快速生长的特点,可非常有效地提取多不饱和脂肪酸。采用超临界流体萃取法(SFE)成功分离了WL-1021中的PUFA。采用高效液相色谱(HPLC)、气相色谱/红外光谱(GC/FT-IR)和气相色谱/质谱(GC/MS)测定了SFE工艺参数,包括压力、温度和培养温度的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Separation of biosynthetic polyunsaturated fatty acid (PUFA) with supercritical fluid.

The separation of biosynthetic polyunsaturated fatty acid (PUFA) was undertaken with supercritical fluid. The polyunsaturated fatty acid was synthesised by WL-1021, which is a strain of marine bacterium isolated from marine fish. The polyunsaturated fatty acid can be very efficiently extracted from WL-1021 that has the characteristics of rapid growth in the artificial medium. The PUFA in WL-1021 has been successfully separated by supercritical fluid extraction (SFE). The SFE process parameters including pressure, temperature and culture temperature variations have been measured by High Performance Liquid Chromatography (HPLC), GC/FT-IR and GC/MS.

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