H Takahashi, T Iwata, Y Kitagawa, Y Shoya, R H Takahashi, K Nagashima, T Kurata
{"title":"抗拓扑异构酶I单克隆抗体抑制DNA松弛和HIV-1 cDNA合成。","authors":"H Takahashi, T Iwata, Y Kitagawa, Y Shoya, R H Takahashi, K Nagashima, T Kurata","doi":"10.1089/027245700429882","DOIUrl":null,"url":null,"abstract":"<p><p>Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.</p>","PeriodicalId":55044,"journal":{"name":"Hybridoma","volume":"19 4","pages":"331-4"},"PeriodicalIF":0.0000,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/027245700429882","citationCount":"2","resultStr":"{\"title\":\"Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis.\",\"authors\":\"H Takahashi, T Iwata, Y Kitagawa, Y Shoya, R H Takahashi, K Nagashima, T Kurata\",\"doi\":\"10.1089/027245700429882\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.</p>\",\"PeriodicalId\":55044,\"journal\":{\"name\":\"Hybridoma\",\"volume\":\"19 4\",\"pages\":\"331-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2000-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1089/027245700429882\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Hybridoma\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/027245700429882\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hybridoma","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/027245700429882","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Monoclonal antibodies against topoisomerase I suppressed DNA relaxation and HIV-1 cDNA synthesis.
Human immunodeficiency virus type 1 (HIV-1) virion is known to carry a number of cellular components including cellular topoisomerase I. Previously, we have demonstrated that topoisomerase I enhances HIV-1 cDNA synthesis in reverse transcription (RT) assays in vitro. In the present study, we have produced six monoclonal antibodies (MAbs) against human topoisomerase I. The MAbs suppressed nicking/closing of supercoiled DNA and cDNA synthesis in an endogenous reverse transcription (ERT) assay using a detergent-disrupted HIV-1 virion. Thus, the results suggest that topoisomerase I plays an important role in RNA-directed DNA polymerization.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
