受体酪氨酸磷酸酶CRYPalpha影响生长锥形态。

Journal of neurobiology Pub Date : 2000-08-01
B K Mueller, M M Ledig, S Wahl
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引用次数: 0

摘要

在神经系统发育过程中,受体酪氨酸激酶和受体蛋白酪氨酸磷酸酶在轴突生长和引导过程中协同作用。在发育中的禽类视网膜系统中,表达了许多不同的受体蛋白酪氨酸磷酸酶。它们中的大多数都有未知的功能。视网膜神经节细胞在轴突和生长锥上至少表达该受体家族的三种不同成员:CRYPalpha、cryp2和PTPmu。CRYPalpha与视网膜基底膜和视顶盖中发现的至少两种不同的配体具有异亲性相互作用。为了分析crypalpha -配体相互作用的作用,将视网膜神经节细胞轴突生长在视网膜基底膜(内限制膜)上,并利用受体特异性抗体和受体碱性磷酸酶融合蛋白从受体侧和配体侧阻断受体-配体相互作用。这两种处理都减少了视网膜轴突的平均长度,并诱导基底膜上视网膜神经节细胞生长锥的形态发生了巨大变化,但在层粘连蛋白、n -钙粘蛋白、基质和洗涤剂处理的基底膜上没有发生变化。这些结果表明,CRYPalpha及其配体在视网膜内轴突生长过程中起促生长分子的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The receptor tyrosine phosphatase CRYPalpha affects growth cone morphology.

During development of the nervous system receptor tyrosine kinases and receptor protein tyrosine phosphatases act in a coordinate way during axon growth and guidance. In the developing avian retinotectal system, many different receptor protein tyrosine phosphatases are expressed. Most of them have unknown functions. Retinal ganglion cells express at least three different members of this receptor family on their axons and growth cones: CRYPalpha, CRYP-2 and PTPmu. CRYPalpha interacts heterophilically with at least two different ligands found in the basal membranes of the retina and the optic tectum. To analyze the role of the CRYPalpha-ligand interaction, retinal ganglion cell axons were grown on retinal basal membranes (inner limiting membrane) and the receptor-ligand interaction was blocked from both the receptor side (by receptor specific antibodies) and from the ligand side by using a receptor-alkaline phosphatase fusion protein. Both of these treatments reduced average retinal axon length and induced a dramatic change in morphology of retinal ganglion cell growth cones on basal membranes, but not on other substrates like laminin, N-cadherin, matrigel- and detergent-treated basal membranes. These results suggest that CRYPalpha and its ligand act as growth-promoting molecules during intraretinal axon growth.

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