小鼠和人:杂交瘤和重组抗体

Immunology today Pub Date : 2000-08-01 DOI:10.1016/S0167-5699(00)01668-6

M Little , S.M Kipriyanov , F Le Gall , G Moldenhauer

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引用次数: 176

摘要

在过去的25年中，已经从杂交瘤中产生了数千种小鼠单克隆抗体。同样的技术现在可以用于从转基因小鼠中克隆人类抗体。在哺乳动物细胞、乳汁和植物中表达后，可以获得相当数量的用于各种诊断和治疗应用的全长抗体和重组片段。

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Of mice and men: hybridoma and recombinant antibodies

Thousands of mouse monoclonal antibodies have been produced from hybridomas over the past 25 years. The same technique can now be used to clone human antibodies from transgenic mice. Full-length antibodies and recombinant fragments engineered for various diagnostic and therapeutic applications can be obtained in reasonably large amounts after expression in mammalian cells, milk and plants.

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来源期刊

Immunology today

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