良好定义的基于磺基甜菜碱的统计共聚物作为潜在的抗生素粘附涂层。

A B Lowe, M Vamvakaki, M A Wassall, L Wong, N C Billingham, S P Armes, A W Lloyd
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引用次数: 82

摘要

以铜绿假单胞菌为模型微生物,以人巨噬细胞和3T3小鼠胚胎成纤维细胞为实验对象,研究了新型聚磺基甜菜碱共聚物作为抗生素粘附膜的应用前景。用甲基丙烯酸正丁酯(nBuMA)与10或30 mol % 2-(二甲氨基)甲基丙烯酸乙酯(DMAEMA)进行基团转移共聚,制备了两种分子量分布较窄、性能良好的统计共聚物。用1,3-丙磺酸在温和条件下处理这些前驱体聚合物,得到了磺胺甜菜碱化nBuMA-DMAEMA共聚物(聚[磺胺甜菜碱-stat- nbuma])。质子核磁共振谱和元素微量分析表明,DMAEMA残基在两种共聚物中都发生了衍生化。将磺化的nBuMA-DMAEMA共聚物包覆在聚甲基丙烯酸甲酯(PMMA)圆盘上,并与PMMA的生物粘附性能进行了比较。统计学上显著减少(p
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Well-defined sulfobetaine-based statistical copolymers as potential antibioadherent coatings.

The potential use of novel poly(sulfobetaine) copolymers as antibioadherent coatings was investigated using Pseudomonas aeruginosa as a model microorganism and human macrophages and 3T3 mouse embryonic fibroblasts. Two well-defined statistical copolymers with narrow molecular weight distributions were prepared by group transfer copolymerization of n-butyl methacrylate (nBuMA) with either 10 or 30 mol % 2-(dimethylamino)ethyl methacrylate (DMAEMA). Sulfobetainized nBuMA-DMAEMA copolymers (poly[sulfobetaine-stat-nBuMA]) were obtained by treating these precursor polymers with 1,3-propanesultone under mild conditions. Both proton NMR spectroscopy and elemental microanalyses indicated that essentially all the DMAEMA residues were derivatized in both copolymers. Poly(methyl methacrylate) (PMMA) discs were coated with the sulfobetainized nBuMA-DMAEMA copolymers and the bioadherent properties of these coated materials were compared with those of PMMA. Statistically significantly fewer (p<.05) bacteria, macrophages, and fibroblasts adhered to the poly(sulfobetaine-stat-nBuMA)-coated PMMA than to the uncoated PMMA. The poly(sulfobetaine-stat-nBuMA) copolymer containing the higher proportion (30 mol %) sulfobetainized DMAEMA residues proved to be the more effective antibioadherent coating. The antibioadherent properties of these coating materials may allow the cost-effective production of dirt-resistant, easy to clean work surfaces, bioinert coatings for medical devices, and antifouling coatings for marine, agricultural, and industrial applications.

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