肌醇、LiCl和肝素对正常和免疫缺陷XID小鼠SRBC抗体反应的影响

M L Tyan
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引用次数: 0

摘要

将naïve成年免疫正常和免疫缺陷XID小鼠的脾脏细胞培养在含有羊红细胞(SRBC)的琼脂上,分别添加或不添加肌醇、肌醇、氯化锂或肝素,并在1或2天后测定抗SRBC抗体形成细胞(PFC)的菌落数。结果发现,十分之一浓度的肌醇和三叉肌醇在增加特异性pfc数量方面同样有效。肌醇、三叉肌醇和氯化锂加速了正常小鼠和XID小鼠脾细胞培养中直接灶的出现。当肝素添加到XID脾细胞培养中,PFC在第1天增加;然而,直到1天后,正常小鼠脾细胞的PFC和灶性才增加。将这些药物的组合添加到脾细胞培养中对灶或PFC的产生没有积极或消极的影响。正常小鼠在第3天和第4天腹腔注射肝素和SRBC后,脾脏PFC增加,但在第7天没有增加。结果表明,这些药物通过远端或更可能独立于布鲁顿酪氨酸激酶(BTK)的信号通路,通过增加增殖和/或分泌速率来调节b细胞反应。目前尚不清楚每种药物的作用机制是否相同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of inositol, LiCl, and heparin on the antibody responses to SRBC by normal and immunodeficient XID mice.

Spleen cells from naïve adult immunocompetent and immunodeficient XID mice were cultured on agar containing sheep red blood cells (SRBC) with and without myo-inositol, scyllo-inositol, lithium chloride, or heparin, and after 1 or 2 days the number of colonies of antiSRBC antibody-forming cells (PFC) were determined. It was found that myo-inositol and scyllo-inositol at one-tenth the concentration were equally effective in increasing the number of specific PFC. Myo-inositol, scyllo-inositol, and lithium chloride accelerated the appearance of direct foci in cultures of spleen cells from normal and XID mice. When heparin was added to cultures of XID spleen cells, PFC were found to be increased on Day 1; however, PFC and foci were not increased in cultures of spleen cells from competent mice until 1 day later. The addition of combinations of these agents to cultures of spleen cells had no positive or negative effect on the generation of foci or PFC. Normal mice given heparin intraperitoneally with SRBC had increased splenic PFC on Days 3 and 4 but not on Day 7. The results suggest that these agents modulate B-cell responses by increasing the rate of proliferation and/or secretion through a signaling pathway(s) distal to, or more likely, independent of Bruton's tyrosine Kinase (BTK). It is not clear that the mechanism is the same with each agent.

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