血清IV型胶原溶解活性和明胶酶水平对小鼠RCT肉瘤转移检测的意义。

Cancer detection and prevention Pub Date : 2000-01-01
K Ohmori, M Kanamori, K Yudoh, T Yasuda
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引用次数: 0

摘要

我们通过fitc标记的IV型胶原降解实验和酶谱分析,研究了血清IV型胶原溶解活性和明胶酶水平作为自发性肺转移动物模型转移的诊断指标的有效性。本研究使用了高转移性RCT(+)和低转移性RCT(-)克隆。RCT(+)组的平均血清IV型胶原溶解活性在后肢截肢后2周开始升高,在第3周和第4周分别为0.45和1.29单位/ml。3周时,这些数值显著高于对照组(p < 0.01);4周时P < 0.001)。RCT(+)组肺结节数与血清IV型胶原溶解活性呈正相关(r = 0.89, p < 0.001)。酶谱分析表明,在第3周和第4周时,RCT(+)组的105-kD溶胶活性高于RCT(-)组。因此,IV型胶原溶解活性和血清明胶酶水平可能是检测转移的有价值的标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Significance of serum type IV collagenolytic activities and gelatinase levels for detection of metastasis in murine RCT sarcoma.

We investigated the usefulness of serum type IV collagenolytic activities and gelatinase levels as diagnostic markers of metastasis in the animal model of spontaneous lung metastasis by FITC-labeled type IV collagen degradation assay and zymographic analyses. High-metastatic RCT(+) and low-metastatic RCT(-) clones were used in the present study. The mean serum type IV collagenolytic activity in the RCT(+) group started to increase from two weeks after hind limb amputation, and was 0.45 and 1.29 unit/ml at three and four weeks. These values were significantly higher than those in the control group (p < 0.01 at three weeks; p < 0.001 at four weeks). A correlation between the number of lung nodules and serum type IV collagenolytic activities in the RCT(+) group was found (r = 0.89, p < 0.001). Zymographic analyses indicated that 105-kD gelatinolytic activities of the RCT(+) group were higher than those of the RCT(-) group at three and four weeks. Thus, type IV collagenolytic activities and serum gelatinase levels might be valuable markers for the detection of metastasis.

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