{"title":"尿嘧啶抑制血小板膜糖蛋白IV的再分配和凝血酶诱导的细胞内α -颗粒血栓反应蛋白的释放。","authors":"T Guo, Y Z Zhang, D X Liu, P Zou, D Shen","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation.</p><p><strong>Methods: </strong>Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin.</p><p><strong>Results: </strong>Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent.</p><p><strong>Conclusion: </strong>Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.</p>","PeriodicalId":24002,"journal":{"name":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1999-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dauricine inhibits redistribution of platelet membrane glycoprotein IV and release of intracellular alpha-granule thrombospondin induced by thrombin.\",\"authors\":\"T Guo, Y Z Zhang, D X Liu, P Zou, D Shen\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation.</p><p><strong>Methods: </strong>Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin.</p><p><strong>Results: </strong>Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent.</p><p><strong>Conclusion: </strong>Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.</p>\",\"PeriodicalId\":24002,\"journal\":{\"name\":\"Zhongguo yao li xue bao = Acta pharmacologica Sinica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo yao li xue bao = Acta pharmacologica Sinica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo yao li xue bao = Acta pharmacologica Sinica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的:探讨尿嘧啶(Dau)抑制血小板膜糖蛋白IV (GPIV)重分布及细胞内α -颗粒血小板反应蛋白(TSP)释放对血小板活化的影响。方法:采用流式细胞术检测洗涤血小板中GPIV的表达及凝血酶诱导的TSP的释放。结果:Dau对静息血小板膜上GPIV和TSP无影响，但抑制GPIV向血小板表面的再分布和活化血小板上TSP的释放。GPIV与TSP变化呈显著正相关(r = 0.511, P < 0.01)。Dau的抑制作用似乎不依赖于Ca2+浓度。结论:Dau抑制GPIV的再分布和凝血酶诱导的细胞内α -颗粒血栓反应蛋白的释放。
Dauricine inhibits redistribution of platelet membrane glycoprotein IV and release of intracellular alpha-granule thrombospondin induced by thrombin.
Aim: To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation.
Methods: Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin.
Results: Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent.
Conclusion: Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.
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