晚期糖基化终产物对培养大鼠主动脉平滑肌细胞二酰基甘油信号通路的影响。

N F Liu, J C Yan, X M Huang
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引用次数: 0

摘要

目的:探讨晚期糖基化终产物(AGEP)是否刺激了二酰基甘油(Dia)信号通路,并探讨维生素E和氨基胍对AGEP诱导的大鼠主动脉血管平滑肌细胞(VSMC) Dia水平的影响。方法:采用放射酶法测定AGEP对体外培养VSMC中Dia水平的影响。采用薄层色谱法和放射自显影法对[32P]磷脂酸进行定量测定。结果:与AGEP-BSA孵育的VSMC中Dia水平呈浓度依赖、双相明显升高。早期是快速和短暂的,在15s达到顶峰。后期在10 min达到最大值,衰减缓慢。不同浓度的AGEP-BSA及不同时间AGEP-BSA糖基化后,VSMC内Dia水平均较对照组显著升高。维生素E 50, 100 nmol。L-1阻止agep - bsa刺激的VSMC Dia水平升高,从(940 +/- 43)pmol。L-1到(599 +/- 38),(290 +/- 21)pmol。l - 1。在氨基胍处理AGEP-BSA组中,相同浓度AGEP-BSA(100、200 mg.L-1)孵育的细胞中Dia水平分别降至(260 +/- 8)和(289 +/- 10)pmol。l - 1。早期糖基化低密度脂蛋白(LDL)不影响Dia水平。结论:AGEP对VSMC的Dia信号通路有较强的刺激作用。维生素E和氨基胍可减少Dia的产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of advanced glycosylation end products on diacylglycerol signaling pathway in cultured rat aortic smooth muscle cells.

Aim: To investigate whether diacylglycerol (Dia) signaling pathway is stimulated by advanced glycosylation end products (AGEP) and to determine effect of vitamin E and aminoguanidine on Dia level induced by AGEP in cultured rat aortic vascular smooth muscle cells (VSMC).

Methods: The effect of AGEP on Dia level in cultured VSMC was measured by radio-enzymatic assay. Quantitative measurements of [32P]phosphatidic acid were performed by thin-layer chromatography and autoradiography.

Results: The Dia level in VSMC incubated with AGEP-BSA was markedly increased in a concentration-dependent, biphasic manner. The early phase was rapid and transient, peaking at 15 s. The late phase reached the maximal level at 10 min and decayed slowly. The Dia levels in VSMC exposed to different concentrations of AGEP-BSA and AGEP-BSA glycosylated for various periods were greatly increased compared with control group. Vitamin E 50, 100 nmol.L-1 prevented the AGEP-BSA-stimulated elevation of Dia level in VSMC, from (940 +/- 43) pmol.L-1 to (599 +/- 38), (290 +/- 21) pmol.L-1, respectively. In aminoguanidine-treated AGEP-BSA groups, Dia level in the cells incubated with the same concentration of AGEP-BSA (100, 200 mg.L-1) were decreased to (260 +/- 8) and (289 +/- 10) pmol.L-1, respectively. Early glycosylated low-density lipoproteins (LDL) did not affect Dia level.

Conclusion: AGEP causes a robust stimulation of Dia signaling pathway in VSMC. Vitamin E and aminoguanidine attenuate the production of Dia.

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