酶联免疫吸附试验(ELISA)检测乙型脑炎病毒中和抗体

L Chow, Y Y Yueh, W L Tseng, Y C Wu, S C Wu, C B Horng
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引用次数: 0

摘要

采用竞争性ELISA法检测乙脑中和抗体。根据人血清乙脑抗体与乙脑单克隆抗体(MAb)竞争乙脑抗原的原理,发现3种乙脑单克隆抗体(E3-3、NPF-5和NNN-5)适合用于乙脑中和抗体的竞争性ELISA检测。竞争性ELISA检测29例乙脑确诊血清标本,检测斑块减少中和试验(PRNT)滴度的敏感性为82.1%(23/28)。竞争ELISA法检测E3-3 MAb对乙脑特异性为100%。1995年57例脑炎血凝抑制(HI)效价和1994年37例脑炎PRNT效价与竞争ELISA的相关系数分别为0.744和0.732。将154份健康人血清的竞争ELISA滴度与PRNT滴度进行比较,结果表明竞争ELISA可检测70%的血清,节省了大量的时间和人力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Detection of neutralizing antibodies to Japanese encephalitis virus by enzyme-linked immunosorbent assay (ELISA)].

Competitive ELISA was used for the detection of neutralizing antibody to JE. Based on the principle that human serum JE antibody competed with JE monoclonal antibody (MAb) for JE antigen, it was found that 3 JE MAbs (E3-3, NPF-5 and NNN-5) were suitable for competitive ELISA for the detection of JE neutralizing antibody. The sensitivity of cometitive ELISA for 29 JE confirmed serum specimens with titer of plaque reduction neutralization test (PRNT) was checked to be 82.1% (23/28). The specificity of E3-3 MAb to JE used in competitive ELISA was 100%. Correlation coefficient of JE confirmed cases of 57 hemagglutination inhibition (HI) titers in 1995 and 37 PRNT titers in 1994 compared with competitive ELISA were 0.744 and 0.732, respectively. Compared the competitive ELISA titers of 154 sera of healthy people with PRNT titers, the results showed that 70% of the sera could be detected by competitive ELISA which saved a lot of time and manpower.

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