大鼠精子发生最后阶段顶体的形态发生,特别涉及小管球复合物。

4区 医学 Q2 Agricultural and Biological Sciences
I Tanii, K Yoshinaga, K Toshimori
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引用次数: 9

摘要

我们报道了大鼠精子发生最后阶段顶体形态的超微结构变化,重点讨论了顶体形态发生与小管球体复合体(TBC)发育的关系。在步骤18-19中,前顶体背皮质的电子朗讯区逐渐减少,最后顶体缩窄,体积减小。在顶体收紧的同时,TBC从晚期精子的头部发育,突出到周围的支持细胞中。为了研究顶体内容物在TBC中的掺入情况,采用周期性酸-希夫(PAS)反应和抗顶体单克隆抗体mMN7对第19步精子进行染色。在TBC和顶体中均发现pas反应性和mmn7免疫反应性。顶体向TBC样结构投射,在TBC核心处观察到与顶体密度相似的物质。这些结果表明,TBC在受精前消除了多余的顶体内容物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphogenesis of the acrosome during the final steps of rat spermiogenesis with special reference to tubulobulbar complexes.

We report the ultrastructural changes in acrosome morphology during the final steps of rat spermiogenesis, focusing on the relationship between the acrosome morphogenesis and the tubulobulbar complexes (TBC) development. During steps 18-19, the electron-lucent area in the dorsal cortex of the anterior acrosome gradually diminished, and finally, the acrosome became condensed and reduced its volume. Simultaneously with this tightening up of the acrosome, TBC developed from the head portion of late spermatids, protruding into the surrounding Sertoli cells. To investigate the incorporation of acrosomal contents into TBC, step 19 spermatids were stained by periodic acid-Schiff (PAS) reaction and by using the anti-acrosomal monoclonal antibody mMN7. Both PAS-reactivity and the mMN7-immunoreactivity were found in the TBC, as well as in the acrosome. In addition, the acrosome projected into the TBC-like structure, and materials of a density similar to that of the acrosome were observed in the core of the TBC. These results suggest that the TBC eliminate excess acrosomal contents prior to spermiation.

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来源期刊
Anatomical Record
Anatomical Record Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
4.30
自引率
0.00%
发文量
0
期刊介绍: The Anatomical Record
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