{"title":"FTIR光谱的药理学应用:抗坏血酸诱导的自由基对耐辐射球菌的影响","authors":"Anne-Marie Melin, Annie Perromat, Gérard Déléris","doi":"10.1002/(SICI)1520-6343(1999)5:4<229::AID-BSPY3>3.0.CO;2-I","DOIUrl":null,"url":null,"abstract":"<p>Fourier transform infrared (FTIR) spectroscopy was used as a convenient and easy-to-run method to monitor radical-induced damage on the radiation-resistant <i>Deinococcus radiodurans</i> strain. Increasing concentrations of ascorbic acid added to the culture medium during the stationary phase produced striking changes in the infrared spectra. These changes especially occurred in the 1700–900 cm<sup>−1</sup> region, which is spectroscopically assigned to the amide I and II components, nucleotide bases, phosphodiester backbone and sugar rings, and were correlated with the oxidant effect of ascorbic acid. Thus, FTIR analysis allows a rapid characterization of the changes induced by ascorbic acid in the cell environment, which can be correlated in part with the generation of free radicals. Beyond a critical ascorbic acid concentration of 40 m<i>M</i>, these free radicals can cause severe damage to the biomolecular components, as soon as the antioxidant defenses of the bacterium are overwhelmed. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 229–236, 1999</p>","PeriodicalId":9037,"journal":{"name":"Biospectroscopy","volume":"5 4","pages":"229-236"},"PeriodicalIF":0.0000,"publicationDate":"1999-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/(SICI)1520-6343(1999)5:4<229::AID-BSPY3>3.0.CO;2-I","citationCount":"18","resultStr":"{\"title\":\"Pharmacologic application of FTIR spectroscopy: Effect of ascorbic acid-induced free radicals on Deinococcus radiodurans\",\"authors\":\"Anne-Marie Melin, Annie Perromat, Gérard Déléris\",\"doi\":\"10.1002/(SICI)1520-6343(1999)5:4<229::AID-BSPY3>3.0.CO;2-I\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Fourier transform infrared (FTIR) spectroscopy was used as a convenient and easy-to-run method to monitor radical-induced damage on the radiation-resistant <i>Deinococcus radiodurans</i> strain. Increasing concentrations of ascorbic acid added to the culture medium during the stationary phase produced striking changes in the infrared spectra. These changes especially occurred in the 1700–900 cm<sup>−1</sup> region, which is spectroscopically assigned to the amide I and II components, nucleotide bases, phosphodiester backbone and sugar rings, and were correlated with the oxidant effect of ascorbic acid. Thus, FTIR analysis allows a rapid characterization of the changes induced by ascorbic acid in the cell environment, which can be correlated in part with the generation of free radicals. Beyond a critical ascorbic acid concentration of 40 m<i>M</i>, these free radicals can cause severe damage to the biomolecular components, as soon as the antioxidant defenses of the bacterium are overwhelmed. © 1999 John Wiley & Sons, Inc. Biospectroscopy 5: 229–236, 1999</p>\",\"PeriodicalId\":9037,\"journal\":{\"name\":\"Biospectroscopy\",\"volume\":\"5 4\",\"pages\":\"229-236\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-07-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/(SICI)1520-6343(1999)5:4<229::AID-BSPY3>3.0.CO;2-I\",\"citationCount\":\"18\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biospectroscopy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291520-6343%281999%295%3A4%3C229%3A%3AAID-BSPY3%3E3.0.CO%3B2-I\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biospectroscopy","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/%28SICI%291520-6343%281999%295%3A4%3C229%3A%3AAID-BSPY3%3E3.0.CO%3B2-I","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 18