吲哚酚直接法测定脑脊液中氨。

J R Huizenga, A W Teelken, A Tangerman, A E de Jager, C H Gips, P L Jansen
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引用次数: 12

摘要

用吲哚酚直接法测定脑脊液中氨的含量。结果与酶法进行了比较。方法简便,精密度(变异系数1.6%)和线性度(r = 0.9999, p < 0.001)良好。该方法的回收率非常好(样品内回收率:范围88 ~ 93,中位数93%;样品间回收率:88-93,中位数91%)。在23名无肝脏疾病的神经系统患者中,参考值范围为8至26,中位数为18微米。男女差异无统计学意义(p = 0.5)。用吲哚酚法得到的值与酶法相等(范围9-28,中位数18微米,p = 0.6)。在深度冷冻(-20℃)中储存时,至少1个月的脑脊液氨浓度没有变化。当在4℃(冰箱)中储存时,必须在2天内进行氨测定。在室温下储存脑脊液会导致氨水平人为升高,应避免。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of ammonia in cerebrospinal fluid using the indophenol direct method.

We have determined ammonia in cerebrospinal fluid (CSF) with the indophenol direct method. The results were compared with an enzymatic method. The method is very simple, and precision (coefficient of variation 1.6%) and linearity (r = 0.9999, p < 0.001) of the method are excellent. The recoveries of the method are very good (within-sample recovery: range 88-93, median 93%; between-sample recovery: 88-93, median 91%). In a population of 23 neurological patients not suffering from liver disease, the reference values ranged from 8 to 26, median 18 microM. Males and females did not differ (p = 0.5). The values obtained with the indophenol method were equal to the enzymatic method (range 9-28, median 18 microM, p = 0.6). On storage in the deep freeze (-20 degrees C), there was no change in CSF ammonia concentration for at least 1 mo. When stored at 4 degrees C (refrigerator), ammonia determinations have to be performed within 2 d. CSF storage at room temperature results in artificially elevated ammonia levels and should be avoided.

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