L Zhou, J Wang, S Peng, J Duan, X Cai, M Zou, Q Su
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引用次数: 6
摘要
利用RT-PCR技术从活化的外周血淋巴细胞中分离出不含信号序列的人白细胞介素-17 (il -17)基因，并以糖基化单体的形式在酵母毕赤酵母中高表达。rhIL-17单体刺激小鼠成纤维细胞3T3细胞分泌IL-6，并特异性结合到3T3细胞上的IL-6受体。
High-level expression of human interleukin-17 in the yeast Pichia pastoris.
Human interleukin-17 (hIL-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by RT-PCR, then highly expressed in the yeast Pichia pastoris in the form of the glycosylated monomer. The monomer of rhIL-17 stimulated mouse fibroblast 3T3 cells to secrete IL-6 and was specifically bound to its receptors on 3T3 cells.
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