{"title":"用地高辛标记的RNA探针进行点印迹的极快速RNA检测","authors":"Chih-Yang Huang, Matsuo Kasai, Dennis E. Buetow","doi":"10.1016/S1050-3862(98)00003-5","DOIUrl":null,"url":null,"abstract":"<div><p>An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 4","pages":"Pages 109-112"},"PeriodicalIF":0.0000,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00003-5","citationCount":"10","resultStr":"{\"title\":\"Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes\",\"authors\":\"Chih-Yang Huang, Matsuo Kasai, Dennis E. Buetow\",\"doi\":\"10.1016/S1050-3862(98)00003-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.</p></div>\",\"PeriodicalId\":77142,\"journal\":{\"name\":\"Genetic analysis, techniques and applications\",\"volume\":\"14 4\",\"pages\":\"Pages 109-112\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00003-5\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetic analysis, techniques and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1050386298000035\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386298000035","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Extremely-rapid RNA detection in dot blots with digoxigenin-labeled RNA probes
An RNA dot blot technique is described which uses digoxigenin-labeled antisense RNA probes and a highly-sensitive chemiluminescent substrate. Detection is extremely rapid: 10 s for even low abundance mRNA.
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