人类巨噬细胞对冠状病毒OC43敏感。

4区医学 Q2 Biochemistry, Genetics and Molecular Biology

Advances in experimental medicine and biology Pub Date : 1998-01-01 DOI:10.1007/978-1-4615-5331-1_82

A R Collins

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引用次数: 21

摘要

用人冠状病毒OC43以1-1.5倍的倍数感染成人和脐带血巨噬细胞，清洗两次以去除未结合的病毒。在第1天的上清液中检测到病毒子代，在2-3天达到峰值，7个样本的平均滴度为5 +/- 3.9 x 10(6) pfu/ml。感染巨噬细胞孵育48小时，采用巢式RT-PCR检测病毒RNA。15%的细胞内检测到病毒核衣壳，单克隆抗体对病毒刺突抗原进行表面染色。体外检测巨噬细胞中感染病毒的扩增和病毒RNA和抗原合成，表明对OC43病毒易感性。

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Human macrophages are susceptible to coronavirus OC43.

Adherent adult and cord blood macrophages were infected with human coronavirus OC43 at a multiplicity of 1-1.5 and washed twice to remove unbound virus. Virus progeny was detected in the supernatant on day 1 and peaked at 2-3 days at an average titer of 5 +/- 3.9 x 10(6) pfu/ml from seven samples. Viral RNA was detected by nested set RT-PCR in infected macrophages incubated for 48 hr. Intracellular viral nucleocapsid was detected in 15% of the cells and surface staining for viral spike antigen was observed using monoclonal antibodies. Amplification of infectious virus and detection viral RNA and antigen synthesis in macrophages in vitro indicates susceptibility to OC43 virus.

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来源期刊

Advances in experimental medicine and biology 医学-医学：研究与实验

CiteScore

5.90

自引率

0.00%

发文量

465

审稿时长

2-4 weeks

期刊介绍： Advances in Experimental Medicine and Biology provides a platform for scientific contributions in the main disciplines of the biomedicine and the life sciences. This series publishes thematic volumes on contemporary research in the areas of microbiology, immunology, neurosciences, biochemistry, biomedical engineering, genetics, physiology, and cancer research. Covering emerging topics and techniques in basic and clinical science, it brings together clinicians and researchers from various fields.