{"title":"四种商用快速诊断试剂盒鉴定金黄色葡萄球菌的评价。","authors":"J J Wu, T C Chang, H M Chen","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Three rapid latex agglutination kits (Aureus Test, Pastorex Staph-Plus, and StaphAurex) and one hemagglutination test kit (SlidexStaph) were compared with the conventional coagulase test for the identification of Staphyloccus aureus. A total of 192 methicillin-resistant S. aureus (MRSA), 75 methicillin-susceptible S. aureus (MSSA) and 86 coagulase-negative staphylococci and Micrococcus spp. were tested. The sensitivities of Aureus Test, Pastorex Staph-Plus, StaphAurex and SlidexStaph were 98.6, 99.4, 90.8 and 97.3%, respectively. Capsular serotypes of 27 strains of MRSA were also determined, in which 15 (55.6%) were capsular serotype 5, 11 (40.7%) were capsular serotype 8, and 1 (3.7%) was nontypeable. All the commercial agglutination kits showed 100% specificity except StaphAurex which had a specificity of only 96.5%. False-positive reactions were found in the strains of S. hominis, S. warneri and unidentified coagulase-negative staphylococci. These results suggest that Aureus Test, Pastorex Staph-Plus, and SlidexStaph are better than StaphAurex for rapid identification of S. aureus.</p>","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of four commercial rapid diagnostic kits for identifying Staphylococcus aureus.\",\"authors\":\"J J Wu, T C Chang, H M Chen\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three rapid latex agglutination kits (Aureus Test, Pastorex Staph-Plus, and StaphAurex) and one hemagglutination test kit (SlidexStaph) were compared with the conventional coagulase test for the identification of Staphyloccus aureus. A total of 192 methicillin-resistant S. aureus (MRSA), 75 methicillin-susceptible S. aureus (MSSA) and 86 coagulase-negative staphylococci and Micrococcus spp. were tested. The sensitivities of Aureus Test, Pastorex Staph-Plus, StaphAurex and SlidexStaph were 98.6, 99.4, 90.8 and 97.3%, respectively. Capsular serotypes of 27 strains of MRSA were also determined, in which 15 (55.6%) were capsular serotype 5, 11 (40.7%) were capsular serotype 8, and 1 (3.7%) was nontypeable. All the commercial agglutination kits showed 100% specificity except StaphAurex which had a specificity of only 96.5%. False-positive reactions were found in the strains of S. hominis, S. warneri and unidentified coagulase-negative staphylococci. These results suggest that Aureus Test, Pastorex Staph-Plus, and SlidexStaph are better than StaphAurex for rapid identification of S. aureus.</p>\",\"PeriodicalId\":24009,\"journal\":{\"name\":\"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of four commercial rapid diagnostic kits for identifying Staphylococcus aureus.
Three rapid latex agglutination kits (Aureus Test, Pastorex Staph-Plus, and StaphAurex) and one hemagglutination test kit (SlidexStaph) were compared with the conventional coagulase test for the identification of Staphyloccus aureus. A total of 192 methicillin-resistant S. aureus (MRSA), 75 methicillin-susceptible S. aureus (MSSA) and 86 coagulase-negative staphylococci and Micrococcus spp. were tested. The sensitivities of Aureus Test, Pastorex Staph-Plus, StaphAurex and SlidexStaph were 98.6, 99.4, 90.8 and 97.3%, respectively. Capsular serotypes of 27 strains of MRSA were also determined, in which 15 (55.6%) were capsular serotype 5, 11 (40.7%) were capsular serotype 8, and 1 (3.7%) was nontypeable. All the commercial agglutination kits showed 100% specificity except StaphAurex which had a specificity of only 96.5%. False-positive reactions were found in the strains of S. hominis, S. warneri and unidentified coagulase-negative staphylococci. These results suggest that Aureus Test, Pastorex Staph-Plus, and SlidexStaph are better than StaphAurex for rapid identification of S. aureus.
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