热处理对水藻CH3氢化酶的部分纯化。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1994-08-01

S H Wang, P C Chen

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引用次数: 0

摘要

在4℃和空气中，水藻的甲基紫原依赖性氢化酶CH3是不稳定的。所有的净化过程都在25摄氏度和氩气气氛下进行。经热处理、deae -纤维素离子交换层析、tsk - fractol凝胶过滤等步骤对酶进行部分纯化。实验结果表明，热处理工艺有利于酶与藻胆蛋白的分离。

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Partial purification of hydrogenase from Anabaena sp. CH3 with heat treatment.

The methylviologen-dependent hydrogenase of Anabaena sp. CH3 is unstable at 4 degrees C and in air. All the purifying procedures were carried out at 25 degrees C and under argon atmosphere. The enzyme was partially purified by the following steps: heat treatment, DEAE-cellulose ion-exchange chromatography and gel filtration on TSK-Fractogel. Experimental results indicated that the heat-treatment procedure was beneficial for the separation of the enzyme from phycobiliproteins.

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来源期刊

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology

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