利用细菌Alcanivorax borkumensis从烷烃中提取含糖脂的新型甘氨酸

Wolf-Rainer Abraham, Holger Meyer, Misha Yakimov
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引用次数: 116

摘要

利用质谱(MS)和核磁共振(NMR)技术对产表面活性剂的烃类细菌Alcanivorax borkumensis的极性脂质进行了分离鉴定。这种生物表面活性剂是一种带有四聚氧酰基侧链的阴离子葡萄糖脂质。从细胞壁中提取的糖脂由这种生物表面活性剂n端与甘氨酸酯化而成。鉴定了10种不同的脂质衍生物,并通过质谱分析分析了它们的结构。它们的变化取决于四种β-羟基脂肪酸(C6、C8和C10)中的一种或两种的链长,以及这些不同脂肪酸在分子中的位置。所有化合物均为首次报道。除了这些糖脂外,还鉴定了三种不同的磷脂酰甘油。虽然这些脂质在所有菌株中都发现,但不同菌株之间不同脂质的相对丰度不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Novel glycine containing glucolipids from the alkane using bacterium Alcanivorax borkumensis

The polar lipids from the hydrocarbon using and biosurfactant-producing bacterium Alcanivorax borkumensis were isolated and identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The biosurfactant produced by this species is an anionic glucose lipid with a tetrameric oxyacyl side chain. The glycolipids extracted from the cell wall consist of this biosurfactant N-terminally esterified with glycine. Ten different derivatives of this lipid type were identified and their structures elucidated by MSMS. They vary by the chain length of one or two of the four β-hydroxy fatty acids (C6, C8 and C10) and by the location of these different fatty acids within the molecule. All compounds are reported here for the first time. In addition to these glycolipids, three different phosphatidylglycerols were identified. While these lipids were found in all strains of A. borkumensis, the relative abundances of the different lipids vary between the strains.

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