Salmo salar cosmid克隆中一个新的假定SINE序列的鉴定。

Journal of marine biotechnology Pub Date : 1998-08-01
Lundin, Mikkelsen, Syed
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引用次数: 0

摘要

在随机选择的一种萨尔鱼(Salmo salar)克隆中发现了一个新的假定的SINE序列。该序列(cSSML032Alu, GenBank登录号:L77085)在212 bp处有78%的同源性,与Salvelinus namaycush AluI卫星序列(GenBank登录号:U27096)在197 bp处有69%的同源性,与S. namaycush AluI卫星序列(GenBank登录号:U27091)同源性较高。cSSML032Alu与一个鲑鱼种群文库的群体杂交表明,它在基因组中分散存在,拷贝数在几千个范围内。在S. salar cSSML032Alu序列中发现的9个碱基的直接末端重复序列和一个潜在的多聚(a)尾残基表明可能存在一个古老的反转录子单元。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of a novel putative SINE sequence in a Salmo salar cosmid clone.

A novel putative SINE sequence was found in a randomly selected cosmid clone of Salmo salar. The sequence (cSSML032Alu, GenBank accession number: L77085) was found to have 78% identity in 212 bp, with one Salvelinus namaycush AluI satellite sequence (GenBank accession number: U27096) and 69% identity in 197 bp, with another AluI satellite sequence from S. namaycush (GenBank accession number: U27091). Colony hybridization of cSSML032Alu to a salmon cosmid library indicated its dispersed presence in the genome, with a copy number in the range of a few thousand. Direct terminal repeats of nine bases and a potential poly(A) tail remnant identified in the S. salar cSSML032Alu sequence indicate a possible ancient retroposon unit.

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