13(S)-羟基十八二烯酸在生物样品中的存在

S.A Spindler , K.S Clark , M.L Blackburn , A.W Bull , R.G Reddy
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引用次数: 2

摘要

亚油酸的氧合代谢物13(S)-羟基十八烯二烯酸最近被证明在细胞调节中起作用。为了在生物系统中检测这种分子,我们最近开发了一种特异性的多克隆抗体。利用该抗体,我们首次通过特异性、敏感性和快速的酶免疫分析法报道了13(S)-羟基十八烯二酸在人尿、细胞培养基和未经处理的山羊血清中的存在。此外,酶联免疫吸附测定数据通过相同样品的气相色谱/质谱分析进行验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Occurrence of 13(S)-Hydroxyoctadecadienoic Acid in Biological Samples

The oxygenated metabolite of linoleic acid, 13(S)-hydroxyoctadecadienoic acid has recently been shown to play a role in cellular regulation. To detect this molecule in biological systems, we recently developed a specific polyclonal antibody. Using this antibody, we report the presence of 13(S)-hydroxyoctadecadienoic acid in human urine, cell culture media, and untreated goat serum for the first time by a specific, sensitive, and rapid enzyme immunoassay. Furthermore, the enzyme linked immunosorbent assay data are verified by gas chromatography/mass spectrometry analysis of the same samples.

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