来自埃利希腹水肿瘤细胞的抗速尿结合蛋白抗体可抑制蛙视网膜色素上皮中Na+、K+、Cl-的共转运。

M la Cour, A Baekgaard, T Zeuthen
{"title":"来自埃利希腹水肿瘤细胞的抗速尿结合蛋白抗体可抑制蛙视网膜色素上皮中Na+、K+、Cl-的共转运。","authors":"M la Cour,&nbsp;A Baekgaard,&nbsp;T Zeuthen","doi":"10.1111/j.1600-0420.1997.tb00399.x","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>To investigate whether antibodies against a 100 kDa protein purified by furosemide affinity chromatography from Ehrlich ascites tumour cells could inhibit Na+, K+, Cl- co-transport in the isolated frog retinal pigment epithelium.</p><p><strong>Methods: </strong>The rate of Na+, K+, Cl- co-transport across the retinal membrane in the isolated frog RPE preparation was measured as the rate of decrease in the intracellular Cl- activity observed after administration of furosemide in the apical bath. The intracellular Cl- activity was measured with double barrelled Cl- sensitive microelectrodes.</p><p><strong>Results: </strong>Incubation of frog retinal pigment epithelium for 30 min with antibodies reduced the rate of Na+, K+, Cl- co-transport by 43%, while leaving all other measured electrophysiological parameters intact.</p><p><strong>Conclusion: </strong>The antibodies inhibit Na+,K+,Cl- co-transport in the frog retinal pigment epithelium. This could be due to binding of the antibodies to the co-transporter itself or to a regulatory protein.</p>","PeriodicalId":7152,"journal":{"name":"Acta ophthalmologica Scandinavica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1600-0420.1997.tb00399.x","citationCount":"5","resultStr":"{\"title\":\"Antibodies against a furosemide binding protein from Ehrlich ascites tumour cells inhibit Na+, K+, Cl- co-transport in frog retinal pigment epithelium.\",\"authors\":\"M la Cour,&nbsp;A Baekgaard,&nbsp;T Zeuthen\",\"doi\":\"10.1111/j.1600-0420.1997.tb00399.x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To investigate whether antibodies against a 100 kDa protein purified by furosemide affinity chromatography from Ehrlich ascites tumour cells could inhibit Na+, K+, Cl- co-transport in the isolated frog retinal pigment epithelium.</p><p><strong>Methods: </strong>The rate of Na+, K+, Cl- co-transport across the retinal membrane in the isolated frog RPE preparation was measured as the rate of decrease in the intracellular Cl- activity observed after administration of furosemide in the apical bath. The intracellular Cl- activity was measured with double barrelled Cl- sensitive microelectrodes.</p><p><strong>Results: </strong>Incubation of frog retinal pigment epithelium for 30 min with antibodies reduced the rate of Na+, K+, Cl- co-transport by 43%, while leaving all other measured electrophysiological parameters intact.</p><p><strong>Conclusion: </strong>The antibodies inhibit Na+,K+,Cl- co-transport in the frog retinal pigment epithelium. This could be due to binding of the antibodies to the co-transporter itself or to a regulatory protein.</p>\",\"PeriodicalId\":7152,\"journal\":{\"name\":\"Acta ophthalmologica Scandinavica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/j.1600-0420.1997.tb00399.x\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta ophthalmologica Scandinavica\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/j.1600-0420.1997.tb00399.x\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta ophthalmologica Scandinavica","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/j.1600-0420.1997.tb00399.x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

摘要

目的:研究从埃利希腹水肿瘤细胞中纯化的抗100 kDa蛋白的抗体是否能抑制离体青蛙视网膜色素上皮中Na+、K+、Cl-的共转运。方法:用尖泡中给予速尿后观察细胞内Cl-活性的降低率来测定离体蛙RPE制剂中Na+、K+、Cl-在视网膜膜上的共转运率。用双管Cl敏感微电极测定细胞内Cl活性。结果:蛙视网膜色素上皮与抗体孵育30分钟,使Na+、K+、Cl-共转运率降低43%,而其他电生理参数保持不变。结论:该抗体可抑制蛙视网膜色素上皮内Na+、K+、Cl-的共转运。这可能是由于抗体与共转运体本身或调节蛋白结合所致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibodies against a furosemide binding protein from Ehrlich ascites tumour cells inhibit Na+, K+, Cl- co-transport in frog retinal pigment epithelium.

Purpose: To investigate whether antibodies against a 100 kDa protein purified by furosemide affinity chromatography from Ehrlich ascites tumour cells could inhibit Na+, K+, Cl- co-transport in the isolated frog retinal pigment epithelium.

Methods: The rate of Na+, K+, Cl- co-transport across the retinal membrane in the isolated frog RPE preparation was measured as the rate of decrease in the intracellular Cl- activity observed after administration of furosemide in the apical bath. The intracellular Cl- activity was measured with double barrelled Cl- sensitive microelectrodes.

Results: Incubation of frog retinal pigment epithelium for 30 min with antibodies reduced the rate of Na+, K+, Cl- co-transport by 43%, while leaving all other measured electrophysiological parameters intact.

Conclusion: The antibodies inhibit Na+,K+,Cl- co-transport in the frog retinal pigment epithelium. This could be due to binding of the antibodies to the co-transporter itself or to a regulatory protein.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信