单个核细胞中上皮来源恶性细胞的检测。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

W Gebauer, E Fallgren Gebauer, C Corbes, S Janssen, F Schunter

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引用次数: 0

摘要

用于敏感性实体瘤大剂量化疗后支持治疗的干细胞分离产品中污染恶性细胞的定性和定量检测是实验室的一项艰巨任务。采用多色流式细胞术和免疫细胞化学对不同浓度的人乳腺癌细胞进行了定量分析。比较特异性、敏感性和实验室处理时间，免疫细胞化学检测上述上皮来源的恶性细胞被证明是优越的方法。

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Detection of malignant cells of epithelial origin in mononuclear cell fractions.

Qualitative and quantitative detection of contaminating malignant cells in stem cell apheresis products intended for supportive therapy after high-dose chemotherapy of sensitive solid tumors is a demanding task for the laboratory. Human breast cancer cells in differing concentrations among mononuclear cells were quantitated by multicolor flow cytometry and immunocytochemistry. Comparing specificity, sensitivity, and laboratory processing time, the immunocytochemical detection of the above-mentioned malignant cells of epithelial origin was demonstrated to be the superior method.

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Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine

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