Per-Johan Jakobsson , Kylie A. Scoggan , James Yergey , Joseph A. Mancini , Anthony W. Ford-Hutchinson
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引用次数: 7
摘要
Western blot检测微粒体GST-II和LTC4合成酶的蛋白表达。这两种酶的17 kDa条带与LTC4形成之间存在相关性。微粒体GST-II的表达效率比LTC4合成酶的表达效率高数倍。除了催化LTC4的生物合成外，微粒体GST-II还产生另一种产物，该产物已被质谱分析。分析表明，新产物是LTC4的同分异构体。
Characterization of microsomal GST-II by Western blot and identification of a novel LTC4 isomer
Protein expression of microsomal GST-II and LTC4 synthase was analyzed by Western blot. Correlation between a 17 kDa band and LTC4 formation was observed for both enzymes. The expression of microsomal GST-II was several fold more efficient than the expression of LTC4 synthase. In addition to catalyzing the biosynthesis of LTC4, microsomal GST-II also produces another product, which has been subjected to mass spectrometric analysis. This analysis demonstrates that the novel product is an isomer of LTC4.
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