厌氧纤维素分解细菌的基因操作。

SAAS bulletin, biochemistry and biotechnology Pub Date : 1997-01-01

K L Anderson

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引用次数: 0

摘要

将转座子Tn1545引入瘤胃纤维素分解菌(Eubacterium cellulosolvens)的染色体中。这是通过以每10(4)个受体细胞中约1个的频率从贝氏梭菌偶联转移转座子实现的。胞质溶剂转缀合物对四环素和红霉素均有耐药性。E.纤维素溶剂也可以作为Tn1545偶联转移回C. beijerinckii的供体。

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Genetic manipulation of anaerobic cellulolytic bacteria.

Transposon Tn1545 was introduced into the chromosome of the ruminal cellulolytic bacterium Eubacterium cellulosolvens. This was achieved by conjugal transfer of the transposon from Clostridium beijerinckii at a frequency of about 1 per 10(4) recipient cells. Transconjugants of E. cellulosolvens were resistant to both tetracycline and erythromycin. E. cellulosolvens could also serve as a donor for conjugal transfer of Tn1545 back into C. beijerinckii.

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SAAS bulletin, biochemistry and biotechnology

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