{"title":"[18O]血栓烷B3作为内标物的气相色谱-选择离子监测微量测定","authors":"Takanori Hishinuma , Yukiko Nakagawa , Tohru Yamazaki , Seiji Hiraku , Michinao Mizugaki","doi":"10.1016/S0090-6980(97)00056-7","DOIUrl":null,"url":null,"abstract":"<div><p>We devised a simple and effective purification for the microdetermination of thromboxane B<sub>3</sub> (TXB<sub>3</sub>), a hydrolysis product of TXA<sub>3</sub>. [<sup>18</sup>O<sub>2</sub>]TXB<sub>3</sub> was synthesized by the repeated base-catalyzed hydrolysis of methyl ester derivatives in [<sup>18</sup>O]water, to obtain an internal standard (IS) for the gas chromatography/selected ion monitoring (GC/SIM) of TXB<sub>3</sub>. The methyl ester (ME)-methoxime (MO)-dimethylisopropylsilyl (DMIPS) ether derivative was prepared, then GC/SIM was carried out by monitoring the ion at m/z 668 for TXB<sub>3</sub> and that at m/z 672 for IS. A good linear response over the range of 10 pg ∼ 10 ng was demonstrated. We were able to detect the levels of TXB<sub>3</sub> in the medium of human erythroleukemia (HEL) cell cultured with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). This method can be used to determine 3-series thromboxane in biological samples.</p></div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00056-7","citationCount":"3","resultStr":"{\"title\":\"Microdetermination of the Thromboxane B3 Gas Chromatography-Selected-Ion Monitoring Using [18O]Thromboxane B3 as an Internal Standard\",\"authors\":\"Takanori Hishinuma , Yukiko Nakagawa , Tohru Yamazaki , Seiji Hiraku , Michinao Mizugaki\",\"doi\":\"10.1016/S0090-6980(97)00056-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We devised a simple and effective purification for the microdetermination of thromboxane B<sub>3</sub> (TXB<sub>3</sub>), a hydrolysis product of TXA<sub>3</sub>. [<sup>18</sup>O<sub>2</sub>]TXB<sub>3</sub> was synthesized by the repeated base-catalyzed hydrolysis of methyl ester derivatives in [<sup>18</sup>O]water, to obtain an internal standard (IS) for the gas chromatography/selected ion monitoring (GC/SIM) of TXB<sub>3</sub>. The methyl ester (ME)-methoxime (MO)-dimethylisopropylsilyl (DMIPS) ether derivative was prepared, then GC/SIM was carried out by monitoring the ion at m/z 668 for TXB<sub>3</sub> and that at m/z 672 for IS. A good linear response over the range of 10 pg ∼ 10 ng was demonstrated. We were able to detect the levels of TXB<sub>3</sub> in the medium of human erythroleukemia (HEL) cell cultured with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). This method can be used to determine 3-series thromboxane in biological samples.</p></div>\",\"PeriodicalId\":20653,\"journal\":{\"name\":\"Prostaglandins\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00056-7\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0090698097000567\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0090698097000567","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Microdetermination of the Thromboxane B3 Gas Chromatography-Selected-Ion Monitoring Using [18O]Thromboxane B3 as an Internal Standard
We devised a simple and effective purification for the microdetermination of thromboxane B3 (TXB3), a hydrolysis product of TXA3. [18O2]TXB3 was synthesized by the repeated base-catalyzed hydrolysis of methyl ester derivatives in [18O]water, to obtain an internal standard (IS) for the gas chromatography/selected ion monitoring (GC/SIM) of TXB3. The methyl ester (ME)-methoxime (MO)-dimethylisopropylsilyl (DMIPS) ether derivative was prepared, then GC/SIM was carried out by monitoring the ion at m/z 668 for TXB3 and that at m/z 672 for IS. A good linear response over the range of 10 pg ∼ 10 ng was demonstrated. We were able to detect the levels of TXB3 in the medium of human erythroleukemia (HEL) cell cultured with eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). This method can be used to determine 3-series thromboxane in biological samples.
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