酵母金属硫蛋白基因表达对金属和氧化应激的响应

IF 4.2 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 1997-03-01 DOI:10.1006/meth.1996.0423

Xiao-Dong Liu, Dennis J. Thiele

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引用次数: 42

摘要

在生物系统中，金属和氧是化学联系在一起的。金属和氧在酶促反应、代谢和信号转导中起着重要作用;然而，金属和氧反应形成剧毒的氧源自由基。在这篇综述中，我们着重于利用酵母细胞作为单细胞真核模型系统，通过酵母金属硫蛋白基因表达的激活来了解对有毒金属和氧源自由基的感觉和保护反应的基本机制。

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Yeast Metallothionein Gene Expression in Response to Metals and Oxidative Stress

Metals and oxygen are chemically linked in biological systems. Metals and oxygen play important roles in enzymatic reactions, metabolism, and signal transduction; however, metals and oxygen react to form highly toxic oxygen-derived free radical species. In this review we focus on the use of yeast cells, as unicellular eukaryotic model systems, to conduct studies aimed at understanding fundamental mechanisms for the sensation and protective responses to toxic metals and oxygen-derived radicals via the activation of yeast metallothionein gene expression.

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.