基于重复序列的PCR (inter-LINE PCR)在基因组重排分析和不同分类水平上的基因组表征中的应用

Jan Smida , Stefan Leibhard , Anja M. Nickel , Friederike Eckardt-Schupp , Ludwig Hieber
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引用次数: 15

摘要

基于共识线序列的寡核苷酸引物产生了高度可重复性的、物种特异性的PCR产物模式,适用于基因组重排的鉴定和不同分类水平的高等和低等真核生物甚至原核生物的区分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Application of repetitive sequence-based PCR (inter-LINE PCR) for the analysis of genomic rearrangements and for the genome characterization on different taxonomic levels

Oligonucleotide primers derived from consensus LINE-sequences generated highly reproducible, species-specific PCR product patterns suitable for the identification of genomic rearrangements and for the discrimination on different taxonomic levels of higher and lower eukaryotes and even prokaryotes.

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