成年日本鹌鹑卵巢表皮生长因子的免疫组化定位。

The Histochemical Journal Pub Date : 1995-11-01
L Van Nassauw, R De Deurwaerder, A Leeuwesteyn, F Harrisson, M Callebaut
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引用次数: 0

摘要

本文研究了成年日本鹌鹑卵巢中表皮生长因子的免疫组化定位。免疫反应性主要见于血管和脊索的平滑肌细胞、灯刷前卵泡的颗粒细胞、间质细胞、灯刷前卵母细胞的Balbiani复合体和神经节。在发育中的卵泡中,在一些颗粒和鞘细胞、辐射带中,特别是在鞘周的细胞团中也检测到免疫反应性。颗粒中免疫染色细胞的数量在卵泡发生期间减少,在排卵后增加。在卵母细胞的卵浆中,免疫反应性在发育过程中从Balbiani复合体转移到辐射带。这些观察结果支持了表皮生长因子主要作用于分化程度较低的卵泡的假设。提示表皮生长因子对卵巢收缩性有调节作用。最后,在一个卵巢中,我们在发育中的小卵母细胞的卵浆中检测到免疫染色的小体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunohistochemical localization of epidermal growth factor in the ovary of the adult Japanese quail.

The present study focuses on the immunohistochemical localization of epidermal growth factor in the ovary of the adult Japanese quail. Immunoreactivity was predominantly found in the smooth muscle cells of blood vessels and of chordae, in granulosa cells of pre-lampbrush follicles, in interstitial cells, in the Balbiani complex of pre-lampbrush oocytes, and in ganglia. In developing follicles, immunoreactivity was also detected in some granulosa and thecal cells, in the zona radiata, and especially in cell clusters localized in the thecal periphery. The number of immunostained cells in the granulosa decreased during folliculogenesis, and increased after ovulation. In the ooplasm of oocytes, immunoreactivity was shifted from the Balbiani complex to the zona radiata during development. These observations support the hypothesis that epidermal growth factors acts primarily on less differentiated follicles. It is also suggested that epidermal growth factor can modulate ovarian contractility. Finally, in one ovary, we detected immunostained bodies in the ooplasm of small developing oocytes.

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