两种甲状腺髓样癌细胞系体外免疫细胞化学特性的研究。

The Histochemical Journal Pub Date : 1995-11-01
M Zabel, J Seidel, A Kaczmarek, J Surdyk-Zasada, J Grzeszkowiak, A Górny
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引用次数: 0

摘要

对源自甲状腺髓样癌的细胞系,即人TT细胞和大鼠rMTC 6-23细胞进行了免疫细胞化学表征。免疫细胞化学研究辅以超微结构研究,包括超微结构免疫细胞化学,以及对降钙素向培养基分泌的放射免疫学估计。在rMTC 6-23细胞(传代24 ~ 30)中,免疫细胞化学未发现激素存在,这与超微结构水平上缺乏分泌颗粒相对应。在寻找的各种蛋白质中,只有神经元特异性烯醇化酶可以被证明。然而,细胞将降钙素分泌到培养基中。TT细胞(传代145 ~ 160)产生分泌颗粒。颗粒中含有降钙素、降钙素基因相关肽、生长抑素、神经紧张素、乙酰脑啡肽、乙酰脑啡肽、胃泌素释放肽、甲状旁腺激素相关蛋白、嗜铬粒蛋白组功能蛋白和突触素。在TT细胞胞浆中发现的其他功能蛋白包括非特异性烯醇化酶、钙结合蛋白和酪氨酸羟化酶。骨化三醇受体定位于细胞核。标记蛋白定位于细胞质(癌胚抗原)和细胞骨架(α -微管蛋白、细胞角蛋白)。随着培养基中离子钙水平的变化,观察到降钙素分泌的变化以及一些激素和功能蛋白的免疫细胞化学检测能力的变化。TT细胞表现出与降钙素分泌相关的多种激素和功能蛋白的表达。此外,这些细胞的超微结构、免疫细胞化学和分泌特征更接近正常的甲状腺滤泡旁细胞,我们认为这是一种更适合功能研究的模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunocytochemical characterization of two thyroid medullary carcinoma cell lines in vitro.

The immunocytochemical characterization of cell lines originating from thyroid medullary carcinoma, i.e. human TT cells and rat rMTC 6-23 cells, was undertaken. The immunocytochemical studies were supplemented by ultrastructural studies, including ultrastructural immunocytochemistry, and by radioimmunological estimation of calcitonin secretion to the medium. In rMTC 6-23 cells (subcultures 24 to 30), no hormone presence was demonstrated immunocytochemically, which corresponded to the absence of secretory granules at the ultrastructural level. Of various proteins sought, only neuron-specific enolase could be demonstrated. Nevertheless, the cells secreted calcitonin into the medium. TT cells (passages 145 to 160) produced secretory granules. The granules contained calcitonin, calcitonin gene-related peptide, somatostatin, neurotensin, met-enkephalin, leu-enkephalin, gastrin releasing peptide, parathyroid hormone-related protein, functional proteins of the chromogranin group and synaptophysin. Other functional proteins found in the cytosol of TT cells included non-specific enolase, calbindin and tyrosine hydroxylase. Receptor for calcitriol was localized in the cell nucleus. Marker proteins were localized in the cytosol (carcinoembryonic antigen) and in the cell skeleton (alpha-tubulin, cytokeratin). Following changes in ionized calcium levels in the medium, changes in calcitonin secretion and in immunocytochemical detectability of some hormones and functional proteins were observed. TT cells demonstrated the expression of numerous hormones and functional proteins associated with calcitonin secretion. Further, the cells in their ultrastructure, immunocytochemical and secretory characteristics, resemble more closely normal parafollicular cells of the thyroid and, in our opinion, represent a more appropriate model for functional studies.

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