{"title":"大鼠海马苔藓纤维含有胆囊收缩素样免疫反应性。","authors":"J Chandy, J P Pierce, T A Milner","doi":"10.1002/ar.1092430415","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The possibility that mossy fiber endings in the rat hippocampal formation may contain cholecystokinin (CCK) was reexamined.</p><p><strong>Methods: </strong>For this, CCK-immunoreactivity was examined by light and electron microscopy using the avidin-biotin complex method.</p><p><strong>Results: </strong>At the light level, the topographical distribution of perikarya and processes with CCK-like immunoreactivity (CCK-LI) was similar to that previously described by others. Ultrastructural analysis of the dentate gyrus and CA3 region of the hippocampus revealed that some mossy fiber terminals contained CCK-LI most often affiliated with large, dense-core vesicles (DCV). Quantitative analysis revealed that 4-8% of the mossy terminal profiles examined (n = 350) contained CCK-labeled DCVs, which corresponded to 0.03-0.2 labeled DCVs per 100 microns2 of neuropil.</p><p><strong>Conclusions: </strong>The presence of CCK-LI within mossy fibers in the rat suggests that there is less species variability in peptide expression in this pathway than formerly believed.</p>","PeriodicalId":50793,"journal":{"name":"Anatomical Record","volume":"243 4","pages":"519-23"},"PeriodicalIF":0.0000,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/ar.1092430415","citationCount":"10","resultStr":"{\"title\":\"Rat hippocampal mossy fibers contain cholecystokinin-like immunoreactivity.\",\"authors\":\"J Chandy, J P Pierce, T A Milner\",\"doi\":\"10.1002/ar.1092430415\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The possibility that mossy fiber endings in the rat hippocampal formation may contain cholecystokinin (CCK) was reexamined.</p><p><strong>Methods: </strong>For this, CCK-immunoreactivity was examined by light and electron microscopy using the avidin-biotin complex method.</p><p><strong>Results: </strong>At the light level, the topographical distribution of perikarya and processes with CCK-like immunoreactivity (CCK-LI) was similar to that previously described by others. Ultrastructural analysis of the dentate gyrus and CA3 region of the hippocampus revealed that some mossy fiber terminals contained CCK-LI most often affiliated with large, dense-core vesicles (DCV). Quantitative analysis revealed that 4-8% of the mossy terminal profiles examined (n = 350) contained CCK-labeled DCVs, which corresponded to 0.03-0.2 labeled DCVs per 100 microns2 of neuropil.</p><p><strong>Conclusions: </strong>The presence of CCK-LI within mossy fibers in the rat suggests that there is less species variability in peptide expression in this pathway than formerly believed.</p>\",\"PeriodicalId\":50793,\"journal\":{\"name\":\"Anatomical Record\",\"volume\":\"243 4\",\"pages\":\"519-23\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/ar.1092430415\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anatomical Record\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/ar.1092430415\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anatomical Record","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/ar.1092430415","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Rat hippocampal mossy fibers contain cholecystokinin-like immunoreactivity.
Background: The possibility that mossy fiber endings in the rat hippocampal formation may contain cholecystokinin (CCK) was reexamined.
Methods: For this, CCK-immunoreactivity was examined by light and electron microscopy using the avidin-biotin complex method.
Results: At the light level, the topographical distribution of perikarya and processes with CCK-like immunoreactivity (CCK-LI) was similar to that previously described by others. Ultrastructural analysis of the dentate gyrus and CA3 region of the hippocampus revealed that some mossy fiber terminals contained CCK-LI most often affiliated with large, dense-core vesicles (DCV). Quantitative analysis revealed that 4-8% of the mossy terminal profiles examined (n = 350) contained CCK-labeled DCVs, which corresponded to 0.03-0.2 labeled DCVs per 100 microns2 of neuropil.
Conclusions: The presence of CCK-LI within mossy fibers in the rat suggests that there is less species variability in peptide expression in this pathway than formerly believed.
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