利用组织化学技术研究一氧化氮合酶的定位和表达。

The Histochemical Journal Pub Date : 1995-10-01
P J Norris, I G Charles, C A Scorer, P C Emson
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引用次数: 0

摘要

本文综述了各种组织化学技术的最新进展,这些技术可用于福尔马林固定组织切片中一氧化氮合酶的细胞定位和表达。讨论了免疫组织化学和NADPH-diaphorase组织化学技术,并评估了用于原位检测一氧化氮合酶mRNA表达的各种探针和报告器的适用性。图还包括说明技术描述和协议的原位杂交和NADPH-diaphorase组织化学。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Studies on the localization and expression of nitric oxide synthase using histochemical techniques.

This review provides an update on the variety of histochemical techniques available for the cellular localization and expression of nitric oxide synthase in formalin-fixed tissue sections. The techniques of immunohistochemistry and NADPH-diaphorase histochemistry are discussed and the suitability of various types of probes and reporters which are useful for in situ detection of nitric oxide synthase mRNA expression are assessed. Figures are also included which illustrate the techniques described and protocols for in situ hybridization and NADPH-diaphorase histochemistry.

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